In the present study, cyanobacterium isolate CHS1 isolated from Hopar glacier, Pakistan, was analyzed for the first time for cell membrane fatty acids and production of pigments. Sequencing of the 16-23S intergenetic region confirmed identification of the isolate CHS1 as Nodularia spumigena. All chlorophyll and carotenoid pigments were quantified using high-performance liquid chromatography and experiments to test tolerance against a range of physico-chemical conditions were conducted. Likewise, the fatty acid profile of the cell membrane CHS1 was analyzed using gas chromatography and mass spectroscopy. The cyanobacterium isolate CHS1 demonstrated tolerance to 8 g/L% NaCl, 35°C and pH 5–9. The characteristic polyunsaturated fatty acid (PUFA) of isolate CHS1, C18:4, was observed in fatty acid methyl esters (FAMEs) extracted from the cell membrane. CHS1 was capable of producing saturated fatty acids (SFA) (e.g., C16:0), monounsaturated fatty acids (MUFA) (e.g., C18:1) and polyunsaturated fatty acids (e.g., C20:5) in the cell membrane. In this study, we hypothesize that one mechanism of cold adaptation displayed by isolate CHS1 is the accumulation of high amounts of PUFA in the cell membrane.

中文翻译：

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从巴基斯坦霍帕尔冰川分离的抗精神病性蓝藻结节藻CHS1的细胞膜脂肪酸和色素组成。

在本研究中，首次分析了从巴基斯坦Hopar冰川分离的蓝细菌分离物CHS1的细胞膜脂肪酸和色素的产生。该16-23S intergenetic区域的测序证实分离物CHS1作为识别节球spumigena。使用高效液相色谱法对所有叶绿素和类胡萝卜素色素进行定量，并进行了试验以测试对一系列理化条件的耐受性。同样，使用气相色谱法和质谱法分析细胞膜CHS1的脂肪酸谱。蓝细菌分离物CHS1表现出对8 g / L％NaCl，35°C和pH 5–9的耐受性。从细胞膜提取的脂肪酸甲酯（FAME）中观察到了分离物CHS1的特征性多不饱和脂肪酸（PUFA）C18：4。CHS1能够在细胞膜中产生饱和脂肪酸（SFA）（例如C16：0），单不饱和脂肪酸（MUFA）（例如C18：1）和多不饱和脂肪酸（例如C20：5）。在这个研究中，