Analysis of a loquat tree with leaf curl symptoms by deep sequencing revealed a novel virus with a single-stranded RNA genome, for which the name "loquat virus A" (LoVA) was proposed. The complete genome sequence comprised 7553 nucleotides (nt) and an additional poly(A) tail at the 3' terminus. Sequence comparisons of LoVA showed moderate similarity to cherry virus A (CVA), currant virus A (CuVA), and mume virus A (MuVA), which are members of the genus Capillovirus in the family Betaflexiviridae. Phylogenetic analysis of the full-genome nt sequence and replicase-like protein supported the placement of LoVA within the genus Capillovirus. However, it has a distinct genome organization, differing from recognized capilloviruses, as it contains three open reading frames (ORFs), with the coat protein (CP) expressed separately from the replication-associated protein (RP) rather than being encoded in the same ORF. This indicates that LoVA is a novel member of the genus Capillovirus in the family Betaflexiviridae with a distinct genomic organization.

中文翻译：

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lo病毒A（Betaflexiviridae家族成员，具有新颖的基因组结构）的完整核苷酸序列。

通过深度测序分析具有叶卷曲症状的lo树，发现了一种具有单链RNA基因组的新型病毒，为此提出了“ lo病毒A”（LoVA）的名称。完整的基因组序列在3'末端包含7553个核苷酸（nt）和另一个poly（A）尾巴。LoVA的序列比较显示出与樱桃病毒A（CVA），黑加仑病毒A（CuVA）和梅毒A（MuVA）的适度相似性，后者是Betaflexiviridae家族中的毛状病毒属成员。全基因组nt序列和复制酶样蛋白的系统发育分析支持LoVA在毛细管病毒属中的放置。但是，它具有独特的基因组组织，不同于公认的毛状病毒，因为它包含三个开放阅读框（ORF），外壳蛋白（CP）与复制相关蛋白（RP）分开表达，而不是在同一ORF中编码。这表明，LoVA是Betaflexiviridae家族中毛状病毒属的一个新成员，具有独特的基因组结构。