The purpose of this work was to characterize the cellular phenotype in inflammatory infiltrates of fetal tissues from pregnant heifers immunized and experimentally challenged with Neospora caninum. Fetuses from 20 heifers separated into 5 groups were obtained. The experiment was designed as follow: Group A, heifers inoculated intravenously with live tachyzoites of Argentine strain NC-6 (n = 4); Group B heifers inoculated subcutaneously with soluble native antigen from the same strain formulated with immune stimulant complexes (ISCOMs) (n = 4); Group C heifers inoculated with recombinant proteins, rNcSAG1, rNcHSP20, rNcGRA7 formulated with ISCOMs (n = 4), Group D heifers inoculated subcutaneously with sterile phosphate buffered solution (n = 4) and Group E heifers inoculated subcutaneously with antigen-free ISCOMs (n = 4). Experimental challenge was performed at 70 days of gestation and all heifers were euthanized 34 days later. Fetal tissues were taken for histological studies. Inflammatory lesions were observed in brain and lung, and immunhistochemistry was used to identify CD3+, CD20+ and MHC II+ cells. The majority of the cells that infiltrate and circumscribe the lesions in the brain and lung tissue expressed MHC II antigen; varying between 70-90% of the total cellular infiltrate. CD3+ cells were also present within the lesions, contributing to up to 30% of the inflammatory cells. CD20+ cells appeared as a marginal group, in some cases, with a range between 10 and 25%. As expected, the immunolabeling of MHC II + and CD3 + cells in fetal tissues was associated with fetal infection with N. caninum. There were statistically significant differences in the distribution and population of the inflammatory infiltrate in relation to the immunogenic treatment and the type of tissue, with inflammatory cells being markedly less extensive fetuses from group A (dams previously exposed to N. caninum) and in brain tissue. This work showed that Neospora-infection induced MHC II+ and CD3+ cells in bovine fetuses from dams receiving experimental vaccines.

中文翻译：

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用犬新孢子虫免疫和攻击牛的胎儿组织中免疫细胞的表型特征。

这项工作的目的是表征来自用新孢子虫免疫和实验攻击的怀孕小母牛的胎儿组织的炎症浸润中的细胞表型。从分成5组的20个小母牛中获得胎儿。实验设计如下：A组，用阿根廷NC-6菌株（n ＝ 4）的活速殖子静脉内接种小母牛。B组小母牛皮下注射了来自同一菌株的可溶性天然抗原，该抗原由免疫刺激复合物（ISCOM）配制（n = 4）；C组小母牛接种重组蛋白，由ISCOM配制的rNcSAG1，rNcHSP20，rNcGRA7（n = 4），D组小母牛皮下接种无菌磷酸盐缓冲溶液（n = 4）和E组小母牛皮下接种无抗原ISCOM（n = 4）。在妊娠70天时进行实验攻击，并在34天后对所有小母牛实施安乐死。取胎儿组织用于组织学研究。在脑和肺中观察到炎性病变，并使用免疫组织化学鉴定CD3 +，CD20 +和MHC II +细胞。浸润并限制脑和肺组织病变的大多数细胞表达MHC II抗原。在总细胞浸润量的70-90％之间变化。病变内也存在CD3 +细胞，占炎症细胞的30​​％。在某些情况下，CD20 +细胞作为边缘群体出现，范围在10％到25％之间。如预期的那样，胎儿组织中MHC II +和CD3 +细胞的免疫标记与犬新孢子虫感染有关。与免疫原性治疗和组织类型有关，炎性浸润物的分布和种群在统计学上有显着差异，其中炎性细胞的A组胎儿（先前暴露于犬新孢子虫）和脑组织中的胎儿明显较少。 。这项工作表明，新孢子虫感染在接受实验疫苗的大坝中的牛胎儿中诱导了MHC II +和CD3 +细胞。