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Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways.
In Vitro Cellular & Developmental Biology - Animal ( IF 2.1 ) Pub Date : 2019-09-19 , DOI: 10.1007/s11626-019-00396-7
Wen Tian 1 , Xuan-Mei Piao 2 , Cheng-Ri Yin 1 , Xiao-Long Jiang 1 , Hao-Ding Sun 1 , Xiao-Li An 1 , Jun Jiang 1 , Mei-Lan Lian 1
Affiliation  

Bioreactor-cultured adventitious roots (ARs) of the endangered medicinal plant Oplopanax elatus Nakai is a novel alternative plant material. To utilize ARs in the product production, the present study investigated the anti-inflammatory effect of O. elatus ARs. In the in vivo experiment, lipopolysaccharide (LPS)-induced acute lung injury disease model was established and several inflammatory indexes were determined. For the LPS-stimulated mice, after pretreatment of AR crude extract (200 mg/kg), cell infiltration in lungs was decreased, the production of proinflammatory mediators, including nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6, and 1β in the bronchoalveolar lavage fluid was evidently reduced, which indicated that O. elatus ARs had an anti-inflammatory effect. In the in vitro experiment, ethyl acetate (EtOAc) fractions (12.5, 25, and 50 μg/mL) were used to treat LPS-induced peritoneal macrophages (PMs) of mice. The production of NO, prostaglandin E2, TNF-α, IL-6, and IL-1β in LPS-stimulated PMs was obviously inhibited (p < 0.05) after pretreatment with EtOAc fractions, and the expression of the inducible nitric oxide synthase and cyclooxygenase were also suppressed. To clarify the anti-inflammatory mechanism, effects of EtOAc fraction on changes of proteins related to the pathways of mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB) were investigated. The phosphorylation of extracellular regulated protein kinases, c-jun n-terminal kinase, and p38 MAPK in LPS-induced PMs was inhibited after pretreatment of EtOAc fractions. In addition, EtOAc fractions enhanced inhibitor of nuclear factor-kappa B-α expression and decreased nuclear translocation of p65 NF-κB. Thus, EtOAc from O. elatus ARs is involved in regulating MAKP and NF-κB signaling pathways to inhibit LPS-induced inflammation.

中文翻译:

Oplopanax elatus不定根培养物通过抑制MAPK和NF-κB信号通路来抑制LPS诱导的炎症。

濒危药用植物Oplopanax elatus Nakai的生物反应器培养不定根(ARs)是一种新型的替代植物材料。为了在产品生产中利用AR,本研究调查了O.elatus ARs的抗炎作用。在体内实验中,建立了脂多糖(LPS)诱导的急性肺损伤疾病模型,并确定了几种炎症指标。对于LPS刺激的小鼠,AR粗提取物(200 mg / kg)预处理后,肺中的细胞浸润减少,促炎性介质的生成,包括一氧化氮(NO),肿瘤坏死因子(TNF)-α和支气管肺泡灌洗液中的白介素(IL)-6和1β明显降低,表明O. elatus AR具有抗炎作用。在体外实验中 乙酸乙酯(EtOAc)馏分(12.5、25和50μg/ mL)用于治疗LPS诱导的小鼠腹膜巨噬细胞(PM)。用EtOAc馏分预处理后,LPS刺激的PM中NO,前列腺素E2,TNF-α,IL-6和IL-1β的产生受到明显抑制(p <0.05),并且诱导型一氧化氮合酶和环加氧酶的表达也被压制了。为了阐明抗炎机制,研究了EtOAc馏分对与丝裂原活化蛋白激酶(MAPKs)和核因子-κB(NF-κB)通路相关的蛋白质变化的影响。预处理EtOAc馏分后,LPS诱导的PMs中的细胞外调节蛋白激酶,c-jun n-末端激酶和p38 MAPK的磷酸化受到抑制。此外,EtOAc组​​分增强了核因子-κB-α表达的抑制剂,并降低了p65NF-κB的核易位。因此,来自O.elatus ARs的EtOAc参与调节MAKP和NF-κB信号传导途径以抑制LPS诱导的炎症。
更新日期:2019-11-01
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