Dendrobium is one of the largest and most important (ornamentally and medicinally) orchid genera. Tissue culture is now an established method for the effective propagation of members of this genus. This review provides a detailed overview of the Dendrobium micropropagation literature. Through a chronological analysis, aspects such as explant, basal medium, plant growth regulators, culture conditions and final organogenic outcome are chronicled in detail. This review will allow Dendrobium specialists to use the information that has been documented to establish, more efficiently, protocols for their own germplasm and to improve in vitro culture conditions based on the optimized parameters detailed in this review. Not only will this expand the use for mass propagation, but will also allow for the conservation of important germplasm. Information on the in vitro responses of Dendrobium for developing efficient protocols for breeding techniques based on tissue culture, such as polyploidization, somatic hybridization, isolation of mutants and somaclonal variants and for synthetic seed and bioreactor technology, or for genetic transformation, is discussed in this review. This is the first such review on this genus and represents half a decade of literature dedicated to Dendrobium micropropagation.

中文翻译：

---

铁皮石micro微繁：综述。

石end属是最大和最重要的（在观赏和医学上）兰花属之一。现在，组织培养是有效繁殖该属成员的既定方法。这篇评论提供了石end微繁殖文献的详细概述。通过时间顺序分析，详细记录了外植体，基础培养基，植物生长调节剂，培养条件和最终器官发生结果等方面。这次审查将使石end兰专家使用已记录的信息，以更有效地建立其自身种质的操作规程，并根据此审查中详细介绍的优化参数来改善体外培养条件。这不仅将扩大大规模繁殖的用途，而且还将允许保存重要的种质。本文讨论石of的体外反应信息，以开发基于组织培养的繁殖技术的有效方案，例如多倍体化，体细胞杂交，突变体和体细胞克隆变异的分离以及合成种子和生物反应器技术或遗传转化的信息。评论。这是该属的首次此类综述，代表了有关石end微繁殖的六十年文献。