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Genotypic adaptations associated with prolonged persistence of Lactobacillus plantarum in the murine digestive tract.
Biotechnology Journal ( IF 4.7 ) Pub Date : 2013-09-26 , DOI: 10.1002/biot.201200259 Hermien van Bokhorst-van de Veen 1 , Maaike J Smelt , Michiel Wels , Sacha A F T van Hijum , Paul de Vos , Michiel Kleerebezem , Peter A Bron
Biotechnology Journal ( IF 4.7 ) Pub Date : 2013-09-26 , DOI: 10.1002/biot.201200259 Hermien van Bokhorst-van de Veen 1 , Maaike J Smelt , Michiel Wels , Sacha A F T van Hijum , Paul de Vos , Michiel Kleerebezem , Peter A Bron
Affiliation
Probiotic bacteria harbor effector molecules that confer health benefits, but also adaptation factors that enable them to persist in the gastrointestinal tract of the consumer. To study these adaptation factors, an antibiotic-resistant derivative of the probiotic model organism Lactobacillus plantarum WCFS1 was repeatedly exposed to the mouse digestive tract by three consecutive rounds of (re)feeding of the longest persisting colonies. This exposure to the murine intestine allowed the isolation of intestine-adapted derivatives of the original strain that displayed prolonged digestive tract residence time. Re-sequencing of the genomes of these adapted derivatives revealed single nucleotide polymorphisms as well as a single nucleotide insertion in comparison with the genome of the original WCFS1 strain. Detailed in silico analysis of the identified genomic modifications pinpointed that alterations in the coding regions of genes encoding cell envelope associated functions and energy metabolism appeared to be beneficial for the gastrointestinal tract survival of L. plantarum WCFS1. This work demonstrates the feasibility of experimental evolution for the enhancement of the gastrointestinal residence time of probiotic strains, while full-genome resequencing of the adapted isolates provided clues towards the bacterial functions involved. Enhanced gastrointestinal residence is industrially relevant because it enhances the efficacy of the delivery of viable probiotics in situ.
中文翻译:
基因型适应症与植物乳杆菌在鼠消化道中的持久存在有关。
益生菌带有效应分子,不仅有益健康,而且还具有适应因子,使它们能够在消费者的胃肠道中持久存在。为了研究这些适应因素,益生菌模型生物植物乳杆菌WCFS1的抗生素抗性衍生物通过连续饲喂三个持续时间最长的菌落而反复暴露于小鼠消化道。暴露于鼠肠可分离出原始菌株的肠道适应性衍生物,该衍生物表现出延长的消化道停留时间。与原始WCFS1菌株的基因组相比,这些适应性衍生物的基因组的重测序显示出单核苷酸多态性以及单核苷酸插入。对已鉴定的基因组修饰进行的详细计算机分析表明,编码细胞包膜相关功能和能量代谢的基因编码区的改变似乎对植物乳杆菌WCFS1的胃肠道存活有利。这项工作证明了增加益生菌菌株在胃肠道停留时间的实验发展的可行性,而对适应分离株的全基因组重测序提供了有关细菌功能的线索。增强的胃肠道居留在工业上是相关的,因为它增强了就地递送益生菌的功效。
更新日期:2019-11-01
中文翻译:
基因型适应症与植物乳杆菌在鼠消化道中的持久存在有关。
益生菌带有效应分子,不仅有益健康,而且还具有适应因子,使它们能够在消费者的胃肠道中持久存在。为了研究这些适应因素,益生菌模型生物植物乳杆菌WCFS1的抗生素抗性衍生物通过连续饲喂三个持续时间最长的菌落而反复暴露于小鼠消化道。暴露于鼠肠可分离出原始菌株的肠道适应性衍生物,该衍生物表现出延长的消化道停留时间。与原始WCFS1菌株的基因组相比,这些适应性衍生物的基因组的重测序显示出单核苷酸多态性以及单核苷酸插入。对已鉴定的基因组修饰进行的详细计算机分析表明,编码细胞包膜相关功能和能量代谢的基因编码区的改变似乎对植物乳杆菌WCFS1的胃肠道存活有利。这项工作证明了增加益生菌菌株在胃肠道停留时间的实验发展的可行性,而对适应分离株的全基因组重测序提供了有关细菌功能的线索。增强的胃肠道居留在工业上是相关的,因为它增强了就地递送益生菌的功效。