Current methods for studying oligodendrocyte myelination using primary neurons are limited by the time, cost and reproducibility of myelination in vitro. Nanofibers with diameters of >0.4 μm fabricated from electrospinning of liquid polystyrene are suitable scaffolds for concentric membrane wrapping by oligodendrocytes. With the advent of aligned electrospinning technology, nanofibers can be rapidly fabricated, standardized, and configured into various densities and patterns as desired. Notably, the minimally permissive culture environment of fibers provides investigators with an opportunity to explore the autonomous oligodendrocyte cellular processes underlying differentiation and myelination. The simplicity of the system is conducive to monitoring oligodendrocyte proliferation, migration, differentiation and membrane wrapping in the absence of neuronal signals. Here we describe protocols for the fabrication and preparation of nanofibers aligned on glass coverslips for the study of membrane wrapping by rodent oligodendrocytes. The entire protocol can be completed within 2 weeks.

中文翻译：

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使用工程纳米纤维模拟少突胶质细胞髓鞘形成的快速且可重复的测定。

目前使用原代神经元研究少突胶质细胞髓鞘形成的方法受到体外髓鞘形成的时间、成本和可重复性的限制。由液体聚苯乙烯静电纺丝制成的直径 >0.4 μm 的纳米纤维是少突胶质细胞同心膜包裹的合适支架。随着定向静电纺丝技术的出现，纳米纤维可以快速制造、标准化并根据需要配置为各种密度和图案。值得注意的是，纤维的最低允许培养环境为研究人员提供了探索分化和髓鞘形成基础的自主少突胶质细胞过程的机会。该系统的简单性有利于监测少突胶质细胞的增殖、迁移、在没有神经元信号的情况下分化和膜包裹。在这里，我们描述了在玻璃盖玻片上制备和制备纳米纤维的协议，用于研究啮齿动物少突胶质细胞的膜包裹。整个协议可以在 2 周内完成。