Abstract Alkaline phosphatase and acid phosphatase are two major enzymatic measures of osteoblastic and osteoclastic activity, respectively. As a result, the preservation of the enzymes in bone specimens to near in vivo accuracy is essential. Despite standardization of the staining process, several factors related to the storage of blocks and slides before sectioning and staining impact the level of enzymes detected in the tissue. Block condition (intact, faced, or unstained) as well as environment (temperature and length of time in storage) affect alkaline phosphatase preservation while the acid phosphatase enzyme remains unaffected. We conclude that to optimally preserve alkaline phosphatase enzyme, methacrylate-embedded undecalcified murine bones should be stored as intact blocks. After sectioning, the faced blocks should be stored at 4°C for optimal enzyme staining of future sections. Furthermore, it is best to stain sections immediately after sectioning. (The J Histotechnol 31(4):169–173, 2008) Submitted April 28, 2008; accepted with revisions June 11, 2008

中文翻译：

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储存条件对骨中酶活性保存的影响

摘要 碱性磷酸酶和酸性磷酸酶分别是成骨细胞和破骨细胞活性的两种主要酶促指标。因此，将骨标本中的酶保存到接近体内的准确性是必不可少的。尽管染色过程标准化，但切片和染色前与块和载玻片的存储相关的几个因素会影响组织中检测到的酶水平。块状条件（完整、表面或未染色）以及环境（温度和储存时间）会影响碱性磷酸酶的保存​​，而酸性磷酸酶不受影响。我们得出的结论是，为了最佳地保存碱性磷酸酶，甲基丙烯酸酯嵌入的未脱钙的小鼠骨骼应作为完整的块储存。切片后，贴面块应储存在 4°C 下，以便对未来切片进行最佳酶染色。此外，最好在切片后立即染色切片。（The J Histotechnol 31(4):169–173, 2008）2008 年 4 月 28 日提交；2008 年 6 月 11 日修订后接受