There is an ever increasing need to find surfaces that are biocompatible for applications like medical implants and microfluidics-based cell culture systems. The biocompatibility of five different surfaces with different hydrophobicity was determined using gene expression profiling as well as more conventional methods to determine biocompatibility such as cellular growth rate, morphology and the hydrophobicity of the surfaces. HeLa cells grown on polymethylmethacrylate (PMMA) or a SU-8 surface treated with HNO3-ceric ammonium nitrate (HNO3-CAN) and ethanolamine showed no differences in growth rate, morphology or gene expression profiles as compared to HeLa cells grown in cell culture flasks. Cells grown on SU-8 treated with only HNO3-CAN showed almost the same growth rate (36 +/- 1 h) and similar morphology as cells grown in cell culture flasks (32 +/- 1 h), indicating good biocompatibility. However, more than 200 genes showed different expression levels in cells grown on SU-8 treated with HNO3-CAN compared to cells grown in cell culture flasks. This shows that gene expression profiling is a simple and precise method for determining differences in cells grown on different surfaces that are otherwise difficult to find using conventional methods. It is particularly noteworthy that no correlation was found between surface hydrophobicity and biocompatibility.

中文翻译：

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使用DNA芯片确定聚合物表面的生物相容性的全基因组表达谱。

越来越需要找到与医学植入物和基于微流体的细胞培养系统等应用具有生物相容性的表面。使用基因表达谱以及更常规的测定生物相容性的方法（例如细胞的生长速度，表面形态和疏水性）来确定五个具有不同疏水性的不同​​表面的生物相容性。与在细胞培养瓶中培养的HeLa细胞相比，在聚甲基丙烯酸甲酯（PMMA）或经HNO3-硝酸硝酸铵（HNO3-CAN）和乙醇胺处理的SU-8表面上生长的HeLa细胞在生长速率，形态或基因表达谱上均无差异。在仅用HNO3-CAN处理的SU-8上生长的细胞显示出与在细胞培养瓶中生长的细胞（32 +/- 1 h）几乎相同的生长速率（36 +/- 1 h）和相似的形态，表明具有良好的生物相容性。然而，与在细胞培养瓶中生长的细胞相比，在用HNO3-CAN处理的SU-8上生长的细胞中有200多个基因显示出不同的表达水平。这表明基因表达谱分析是一种简单而精确的方法，用于确定生长在不同表面上的细胞中的差异，而使用常规方法很难找到这些差异。特别值得注意的是，在表面疏水性和生物相容性之间未发现相关性。与在细胞培养瓶中生长的细胞相比，用HNO3-CAN处理的SU-8上生长的细胞中有200多个基因显示出不同的表达水平。这表明基因表达谱分析是一种简单而精确的方法，用于确定生长在不同表面上的细胞中的差异，而使用常规方法很难找到这些差异。特别值得注意的是，在表面疏水性和生物相容性之间未发现相关性。与在细胞培养瓶中生长的细胞相比，用HNO3-CAN处理的SU-8上生长的细胞中有200多个基因显示出不同的表达水平。这表明基因表达谱分析是一种简单而精确的方法，用于确定生长在不同表面上的细胞中的差异，而使用常规方法很难找到这些差异。特别值得注意的是，在表面疏水性和生物相容性之间未发现相关性。