The genetic and physiological properties of Acinetobacter baylyi strain ADP1 make it an inviting subject for investigation of the properties underlying its nutritional versatility. The organism possesses a relatively small genome in which genes for most catabolic functions are clustered in several genetic islands that, unlike pathogenicity islands, give little evidence of horizontal transfer. Coupling mutagenic polymerase chain reaction to natural transformation provides insight into how structure influences function in transporters, transcriptional regulators, and enzymes. With appropriate selection, mutants in which such molecules have acquired novel function may be obtained. The extraordinary competence of A. baylyi for natural transformation and the ease with which it expresses heterologous genes make it a promising platform for construction of novel metabolic systems. Steps toward this goal should take into account the complexity of existing pathways in which transmembrane trafficking plays a significant role.

中文翻译：

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拜氏不动杆菌提供了进行遗传研究的机会，这是一种营养丰富的细菌，能够自然转化。

拜氏不动杆菌菌株ADP1的遗传和生理特性使其成为研究其营养通用性的诱人对象。该生物体具有相对较小的基因组，其中大多数分解代谢功能的基因聚集在几个遗传岛上，与致病岛不同，这些遗传岛几乎没有水平转移的迹象。诱变聚合酶链反应与自然转化的耦合提供了有关结构如何影响转运蛋白，转录调节剂和酶功能的见解。通过适当的选择，可以获得这样的分子具有新功能的突变体。A的非凡能力 天然转化的baylyi及其表达异源基因的简便性使其成为构建新型代谢系统的有前途的平台。实现这一目标的步骤应考虑到跨膜贩运在其中发挥重要作用的现有途径的复杂性。