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Escherichia coli growth under modeled reduced gravity.
Microgravity Science and Technology ( IF 1.8 ) Pub Date : 2005-03-17 , DOI: 10.1007/bf02870967
Paul W. Baker , Michelle L. Meyer , Laura G. Leff

Bacteria exhibit varying responses to modeled reduced gravity that can be simulated by clino-rotation. When Escherichia coli was subjected to different rotation speeds during clino-rotation, significant differences between modeled reduced gravity and normal gravity controls were observed only at higher speeds (30-50 rpm). There was no apparent affect of removing samples on the results obtained. When E. coli was grown in minimal medium (at 40 rpm), cell size was not affected by modeled reduced gravity and there were few differences in cell numbers. However, in higher nutrient conditions (i.e., dilute nutrient broth), total cell numbers were higher and cells were smaller under reduced gravity compared to normal gravity controls. Overall, the responses to modeled reduced gravity varied with nutrient conditions; larger surface to volume ratios may help compensate for the zone of nutrient depletion around the cells under modeled reduced gravity.

中文翻译:

在模拟的重力降低下大肠杆菌的生长。

细菌对模拟的重力降低表现出不同的响应,而重力的降低可以通过倾斜旋转来模拟。当大肠杆菌在倾斜旋转过程中经受不同的旋转速度时,仅在较高的速度(30-50 rpm)下,观察到建模的重力降低和正常重力控制之间的显着差异。去除样品对获得的结果没有明显影响。当大肠杆菌在基本培养基(40 rpm)中生长时,细胞大小不受建模的重力降低的影响,并且细胞数量几乎没有差异。但是,在较高的营养条件下(即稀的营养肉汤),与正常重力对照相比,在重力降低的情况下,总细胞数更高,细胞更小。总体而言,对模拟的重力降低的响应随营养条件而变化。
更新日期:2019-11-01
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