Integrins are heterodimeric glycoproteins that have been found to undergo dynamic temporal and spatial changes in the endometrium during the menstrual cycle and in early pregnancy. Specificity of integrins is known to be different in human endometrial stromal cells and decidual cells. These shifts of integrins suggested to play an important role in embryo implantation and can be modulated by progesterone, cAMP derivatives, and cytokines. The mechanisms of decidualization and its precise physiological role are still not clearly understood and in vitro systems could provide an alternative that overcomes limitations of studying such complex biological phenomena in vivo at the time of implantation. This study was undertaken to establish an in vitro model system for human decidualization using 8-bromo-cAMP and to investigate the characteristics of stromal integrin expression in vitro by 8-Br-cAMP. Endometrial stromal cells were isolated and cultured, and then were induced to decidualize by 0.5 mM 8-Br-cAMP for 15 days. Immunofluorescence staining and flow cytometric analyses of the integrin subunits (alpha1, alpha4, alpha5, alpha6, beta1 and alphavbeta3) were performed at day 9. In the presence of 8-Br-cAMP, the staining intensity of alphavbeta3 was significantly higher than control and measurements for alpha1, alpha4, alpha5, alpha6, and beta1 were similar. Immunofluorescent localization of the integrins reflected the differences obtained from the flow cytometric analyses described above. In summary, the expression of alphavbeta3 integrin increased in stromal cells in vitro decidualized by 8-Br-cAMP and this up-regulation of alphavbeta3 integrin expression during decidualization might influence on human implantation.

中文翻译：

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体外8-Br-cAMP诱导蜕膜化的人子宫内膜基质细胞中整合素表达的特征。

整联蛋白是异二聚体糖蛋白，已发现其在月经周期和怀孕初期子宫内膜发生动态的时空变化。已知整联蛋白的特异性在人子宫内膜基质细胞和蜕膜细胞中是不同的。整联蛋白的这些变化表明在胚胎着床中起重要作用，并且可以被孕酮，cAMP衍生物和细胞因子调节。蜕膜化的机制及其确切的生理作用仍不清楚，并且体外系统可以提供一种替代方法，以克服在植入时在体内研究此类复杂生物学现象的局限性。进行这项研究以建立使用8-bromo-cAMP进行人类蜕膜化的体外模型系统，并研究8-Br-cAMP在体外表达基质整合素的特征。分离并培养子宫内膜间质细胞，然后通过0.5 mM 8-Br-cAMP诱导蜕膜化15天。在第9天进行整联蛋白亚基（α1，α4，α5，α6，β1和αvbeta3）的免疫荧光染色和流式细胞仪分析。在存在8-Br-cAMP的情况下，αvbeta3的染色强度显着高于对照和alpha1，alpha4，alpha5，alpha6和beta1的测量结果相似。整联蛋白的免疫荧光定位反映了从上述流式细胞仪分析获得的差异。综上所述，