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Bacteriorhodopsin crystal growth in reduced gravity--results under the conditions, given in CPCF on board of a Space Shuttle, versus the conditions, given in DCAM on board of the Space Station Mir.
Microgravity Science and Technology ( IF 1.8 ) Pub Date : 2002-09-11 , DOI: 10.1007/bf02872073
Ch Zorb 1 , A Weisert , J Stapelmann , G Smolik , D C Carter , B S Wright , K D Brunner-Joos , G Wagner
Affiliation  

For the purpose of bio-electronics, bacteriorhodopsin was crystallized into two habits through liquid-liquid-diffusion, namely individual needles of up to 1.9 mm in length and needle bunch-like clusters of up 4.9 mm in total length. In both the reduced gravity experiments performed, the morphology of the individual needles (crystal form A) had improved in terms of sharp needle edges and compact needle packing, compared to the parallel ground controls. For the long duration wide range low gravity condition in the "Diffusion-controlled Crystallization Apparatus for Microgravity (DCAM)" on Mir (STS-89 up), needle bunches on average were longer there than on the ground, while the compactness of the clusters, i.e. the average ratio of clustered length to clustered width was the reverse. Some exceptionally large individuals needles were grown in DCAM. For the "Commercial Protein Crystallization Facility (CPCF)" in short duration high definition microgravity conditions during a science mission of the Space Shuttle Discovery (STS-95), size and shape of the individual needles were homogeneous and superior to those of both the parallel ground controls and the results in DCAM. In CPCF, the average volume of the individual needles in suspension was increased by 50% in microgravity compared to those in the ground control.

中文翻译:

细菌视紫红质晶体在重力降低的情况下生长-结果是在航天飞机机载CPCF中给出的条件下,而在空间站Mir机载DCAM中给出的条件下得出的。

出于生物电子学的目的,细菌视紫红质通过液-液扩散被结晶成两个习惯,即最长达1.9 mm的单个针和全长达4.9 mm的针束状簇。在进行的两个重力降低实验中,与平行的地面对照相比,单个针头(晶形A)的形态在尖锐的针头边缘和紧凑的针头填充方面得到了改善。对于Mir(STS-89 up)上的“微重力扩散控制结晶设备(DCAM)”中的长时间宽范围低重力条件,那里的针束平均长于地面,而簇的紧密度,即,聚簇长度与聚簇宽度的平均比率是相反的。一些非常大的个体针头在DCAM中生长。在航天飞机发现(STS-95)科学任务期间的短时间内高清晰度微重力条件下的“商业蛋白质结晶设施(CPCF)”中,单个针的大小和形状均一,并且优于并行的两种地面控制和DCAM中的结果。在CPCF中,与地面对照相比,悬浮液中单个针的平均体积在微重力下增加了50%。单个针的大小和形状均一,并且优于平行地面对照和DCAM的结果。在CPCF中,与地面对照相比,悬浮液中单个针的平均体积在微重力下增加了50%。单个针的大小和形状均一,并且优于平行地面对照和DCAM的结果。在CPCF中,与地面对照相比,悬浮液中单个针的平均体积在微重力下增加了50%。
更新日期:2019-11-01
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