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Potential of Burkholderia cepacia RQ1 in the biodegradation of heavy crude oil.
International Microbiology ( IF 3.1 ) Pub Date : 2002 , DOI: 10.1007/s101230100018
A Okoh 1 , S Ajisebutu , G Babalola , M R Trejo-Hernandez
Affiliation  

The potential of Burkholderia cepacia strain RQ1 in the biodegradation of heavy crude oil (Maya) was assessed to develop an active indigenous bacterial consortium for the bioremediation of crude oil-polluted systems in Nigeria. The heavy crude oil (Maya) was utilized as sole source of carbon, attaining maximum cell densities of 108 cfu ml–1 from an initial 105 cfu ml–1 in 15 days. Biomass also increased with oil concentrations up to 0.8% (w/v). Growth rates ranged from 0.028 h–1 to 0.036 h–1 and degradation rates decreased with increasing concentrations of oil from 0.009 day–1 to 0.004 day–1. The quantity of oil metabolized increased significantly (P<0.05) with increasing concentrations of oil. However, the growth of the bacterium was inhibited at crude oil concentrations beyond 6% (w/v). The pH of the culture media also dropped significantly (P<0.05) during the 15-day test period, while the non-asphaltic fractions of the oil were significantly reduced (by about 89%) during the same period. The bacterium harbours a plasmid of about 10 kb that lacks restriction sites for the endonucleases Asp718, BamHI and PstI.

中文翻译:

洋葱伯克霍尔德菌RQ1在重质原油生物降解中的潜力。

评估伯克霍尔德菌洋葱伯克霍尔德菌菌株RQ1在重质原油(Maya)生物降解中的潜力,以开发一个活跃的本土细菌财团,用于对尼日利亚的原油污染系统进行生物修复。重质原油(Maya)被用作唯一的碳源,在15天内从最初的10 5  cfu ml –1达到最大细胞密度10 8  cfu ml –1。随着油浓度高达0.8%(w / v),生物量也会增加。增长率从0.028 h –1到0.036 h –1不等,降解率随着油浓度的增加而降低,从0.009天–1到0.004天–1。随着油浓度的增加,代谢的油量显着增加(P <0.05)。但是,在原油浓度超过6%(w / v)时,细菌的生长受到抑制。在15天的测试期间,培养基的pH值也显着下降(P <0.05),而在同一时期中,油的非沥青成分显着降低(约89%)。该细菌带有一个约10 kb的质粒,该质粒缺少核酸内切酶Asp 718,Bam HI和Pst I的限制性酶切位点。
更新日期:2020-09-22
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