Treated wastewater samples were collected, filtered using sterile 47-mm cellulose nitrate membrane and DNA extracted from the filtered materials. The presence of Blastocystis sp. was confirmed via polymerase chain reaction (PCR) targeting the SSU rRNA gene of Blastocystis sp. in 5/12 of samples. Based on the subtype analysis after sequencing, 2, 2 and 1 of ST2, ST6 and ST8 were detected among the isolates, respectively. Furthermore, both ST6s were allele 139, alleles 11 and 138 were identified in ST2 and the only ST8 was allele 95. The phylogenetic tree showed that one of ST2 was clustered together with those ST2 that were already reported from humans and animals. The presence of Blastocystis sp. in treated wastewater can indicate the potential role of this type of water for irrigation in the transmission of pathogenic microorganisms to downstream farmlands.

中文翻译：

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Blastocystis sp。的分子分析。和其亚型来自通常用于伊朗蔬菜农田灌溉的经处理废水。

收集处理过的废水样品，使用无菌的47毫米硝酸纤维素膜过滤，并从过滤后的材料中提取DNA。芽孢杆菌属的存在。通过靶向Blastocystis sp的SSU rRNA基因的聚合酶链反应（PCR）证实。在5/12的样本中。根据测序后的亚型分析，在分离物中分别检测到ST2，ST6和ST8的2、2和1。此外，两个ST6都是等位基因139，在ST2中鉴定出等位基因11和138，唯一的ST8是等位基因95。系统进化树显示，ST2之一与已经从人和动物中报道的那些ST2聚在一起。芽孢杆菌属的存在。