This study aimed to detect the presence of Acanthamoeba spp. in different water resources of Zahedan, southeast of Iran, and also systematically reviewed all publications regarding Acanthamoeba in Iran (2005-2018). Fifty water samples were collected from different water resources in Zahedan. The positive samples were identified morphologically and subjected to polymerase chain reaction (PCR) using fragments of 18S rRNA. In the systematic review, data collection using particular terms was carried out using the following electronic databases including Science Direct, ISI Web of Science, MEDLINE, EBSCO, Scopus, and Google Scholar. A total of 17 (34%) samples were positive for Acanthamoeba spp., and nucleotide sequencing indicated that 15 samples (88.23%) belonged to the T4 genotype and the rest belonged to the T5 genotype. A total of 39 studies reported genotyping of Acanthamoeba spp. from various geographical areas of Iran and revealed that T4 (35 studies), T5 (19 studies), T3 (11 studies), T11 (8 studies), and T2 (6 studies) genotypes were the most prevalent in Iran. The T4 genotype of Acanthamoeba is a prevalent free-living amoeba and widely distributed not only in Zahedan but also in other provinces of Iran. Phylogenetic analysis reveals that A. castellanii and A. griffini predominantly colocalize with the T4 genotype.

中文翻译：

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棘阿米巴菌株的基因分型测定：伊朗的一项原始研究和系统评价。

这项研究旨在检测棘阿米巴属菌的存在。在伊朗东南部Zahedan的不同水资源中进行研究，还系统地审查了有关伊朗Acanthamoeba的所有出版物（2005-2018年）。从Zahedan的不同水资源中收集了50个水样。对阳性样品进行形态学鉴定，并使用18S rRNA片段进行聚合酶链反应（PCR）。在系统审查中，使用以下电子数据库（包括Science Direct，ISI Web of Science，MEDLINE，EBSCO，Scopus和Google Scholar）进行了使用特定术语的数据收集。总共17个样品（34％）的棘阿米巴菌属呈阳性，核苷酸测序表明15个样品（88.23％）属于T4基因型，其余样品属于T5基因型。共有39项研究报告了棘阿米巴属菌种的基因型。来自伊朗各个地理区域的数据表明，T4（35个研究），T5（19个研究），T3（11个研究），T11（8个研究）和T2（6个研究）基因型在伊朗最为普遍。棘阿米巴的T4基因型是一种普遍存在的自由生存的变形虫，不仅广泛分布在扎赫丹，而且在伊朗其他省份也广泛分布。系统发育分析表明，A。castellanii和A. griffini主要与T4基因型共定位。棘阿米巴的T4基因型是一种普遍存在的自由生存的变形虫，不仅在Zahedan而且在伊朗其他省份也广泛分布。系统发育分析表明，A。castellanii和A. griffini主要与T4基因型共定位。棘阿米巴的T4基因型是一种普遍存在的自由生存的变形虫，不仅广泛分布在扎赫丹，而且还分布在伊朗的其他省份。系统发育分析表明，A。castellanii和A. griffini主要与T4基因型共定位。