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Effects of 2,2-dichloroacetamide (DCAcAm), an emerging disinfection by-product in drinking water, on the intestinal microbiota of adult zebrafish.
Journal of Water & Health ( IF 2.3 ) Pub Date : 2019-10-23 , DOI: 10.2166/wh.2019.081
Bin Xue 1 , Chenyu Li 1 , Shang Wang 1 , Chen Zhao 1 , Kun Dai 2 , Wenhong Li 1 , Zhuge Xi 1 , Jingfeng Wang 1 , Zhigang Qiu 1 , Zhiqiang Shen 1
Affiliation  

The presence of disinfection by-products (DBPs) increases the mutagenicity of water and may pose adverse health effects. Gut microbiota exerts a fundamental role on host physiology, and how extrinsic perturbations influence its composition has been increasingly examined. However, the effect of DBPs on gut microbiota is still poorly understood. In the present study, adult zebrafish were exposed to different concentrations of dichloroacetamide (DCAcAm, an emerging nitrogenous DBP) for 30 days. Sequencing of 16S rRNA amplicons revealed a significant change in the richness and diversity of microbiota in the gut of DCAcAm-exposed zebrafish. At the phylum level, the abundance of Proteobacteria decreased and the abundance of Fusobacteria and Firmicutes increased significantly in the gut after exposure to 100 and 500 μg/L DCAcAm. At the genus level, the abundances of several bacteria which are considered pathogens or opportunistic pathogens in fish and closely related to fish metabolism, disease and inflammation (Aeromonas, Stenotrophomonas, Bacteroides and Ralstonia) increased in the DCAcAm-treated groups. Our results reveal that DBPs in drinking water potentially affect gut microbiota composition, which may contribute to the toxicity assessment of DBPs in future and provide new insight into the complex interactions between the DBPs in drinking water and host health.

中文翻译:

饮用水中一种新兴的消毒副产物2,2-二氯乙酰胺(DCAcAm)对成年斑马鱼肠道菌群的影响。

消毒副产物(DBP)的存在会增加水的致突变性,并可能对健康造成不利影响。肠道菌群对宿主生理起着重要作用,并且越来越多地研究了外部干扰如何影响其组成。然而,DBPs对肠道菌群的影响仍知之甚少。在本研究中,成年斑马鱼暴露于不同浓度的二氯乙酰胺(DCAcAm,一种新兴的含氮DBP)中达30天。16S rRNA扩增子的测序表明,暴露于DCAcAm的斑马鱼肠道中微生物群的丰富度和多样性发生了显着变化。在门区域,暴露于100和500μg/ L DCAcAm后,肠道中的变形杆菌数量减少,而富集的细菌和硬菌增加。在属上 DCAcAm治疗组中被认为是鱼类病原体或机会病原体且与鱼类代谢,疾病和炎症密切相关的几种细菌(气单胞菌,嗜单胞菌,拟杆菌和罗氏菌)的丰富度增加。我们的结果表明,饮用水中的DBP可能会影响肠道菌群的组成,这可能有助于将来DBP的毒性评估,并为饮用水中DBP与宿主健康之间的复杂相互作用提供新的见解。
更新日期:2019-11-01
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