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Effect of magnetic field exposure on calcium channel currents using patch clamp technique.
Bioelectromagnetics ( IF 1.9 ) Pub Date : 2002-04-12 , DOI: 10.1002/bem.10022
Mayumi Obo 1 , Shiro Konishi , Yoshihisa Otaka , Satoshi Kitamura
Affiliation  

Calcium influxes through the membrane of PC-12D cells were measured under exposure to DC biased AC magnetic fields in resonant conditions of the ion cyclotron and the ion parametric resonance hypotheses and compared with influxes in cells without exposure to the magnetic field. After cancellation of the geomagnetic field, the cells were exposed to the horizontal fields generated by a current sheet, a planar sheet of conductor which generated a satisfactorily homogeneous horizontal magnetic field on the stage of a microscope without hindering treatment of a cell under observation. At or near any resonant conditions, no change in calcium influx could be detected under standard patch clamp conditions.

中文翻译:

使用膜片钳技术,磁场暴露对钙通道电流的影响。

在离子回旋加速器的共振条件下和离子参量共振假设的条件下,在暴露于直流偏置的交流磁场下,测量通过PC-12D细胞膜的钙流入量,并与未暴露于磁场的细胞中的钙流入量进行比较。在消除地磁场之后,将细胞暴露于由电流片产生的水平场,该电流片为在导体平台上产生令人满意的均匀水平磁场的平面导体片,而不会妨碍对观察细胞的处理。在任何共振条件或接近共振条件的情况下,在标准膜片钳条件下都无法检测到钙流入的变化。
更新日期:2019-11-01
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