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50 Hz magnetic fields of varying flux intensity affect cell shape changes in invertebrate immunocytes: the role of potassium ion channels.
Bioelectromagnetics ( IF 1.9 ) Pub Date : 2002-04-12 , DOI: 10.1002/bem.10021
Enzo Ottaviani 1 , Davide Malagoli , Alex Ferrari , Davide Tagliazucchi , Angela Conte , Fabriziomaria Gobba
Affiliation  

The effect induced by exposure to 50 Hz magnetic fields (MFs) in immunocytes from the mussel Mytilus galloprovincialis is evaluated. The whole animal was exposed for 15 and 30 min to MF intensities ranging from 200 to 1,000 microT. The changes in the cellular shape of immunocytes, expressed as shape factor (SF), were studied at different times after addition of the chemotacting substance N-formyl-Meth-Leu-Phe (fMLP). Results show that MFs provoke differing delays in fMLP-induced cellular shape changes: 200 microT are ineffective, while levels from 300 microT upwards cause a significant increase in immunocyte SF values compared to controls. Reactivation of the cells is possible up to an intensity of 600 microT. The use of PCO 400, an opener of ATP-sensitive K+ channels, shows that potassium channels are involved in the effect of MFs on M. galloprovincialis immunocytes.

中文翻译:

不同通量强度的50 Hz磁场会影响无脊椎动物免疫细胞的细胞形状变化:钾离子通道的作用。

评估了贻贝贻贝贻贝的免疫细胞暴露于50 Hz磁场(MFs)诱导的效果。将整个动物暴露于200至1,000 microT的MF强度下15和30分钟。加入趋化性物质N-甲酰基-甲基-Leu-Phe(fMLP)后,在不同时间研究了免疫细胞的细胞形状变化,以形状因子(SF)表示。结果表明,MFs在fMLP诱导的细胞形状变化中引起不同的延迟:200 microT无效,而300 microT向上的水平导致免疫细胞SF值与对照组相比显着增加。细胞重新激活的强度可能高达600 microT。使用PCO 400(ATP敏感的K +通道的开放剂)表明钾通道参与了MF对M的作用。
更新日期:2019-11-01
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