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Anticancer Activities of Cu(II) Complex-Schiff Base and Low-Frequency Electromagnetic Fields and the Interaction Between Cu(II) Complex-Schiff Base with Bovine Serum Albumin by Spectroscopy.
Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2019-10-24 , DOI: 10.1007/s12010-019-03118-7
Soheyla Yadamani 1 , Ali Neamati 1 , Masoud Homayouni-Tabrizi 1 , Samira Yadamani 1 , Afsaneh Javdani-Mallak 1 , S Ali Beyramabadi 2
Affiliation  

Cancer is the consequence of abnormal cell proliferation, which leads to the formation of abnormal mass. In this study, we aimed to determine the anticancer properties of Cu(II)-Schiff base complex and low-frequency electromagnetic field, and the interaction between BSA and Cu(II) complex. Firstly, Schiff base of the Cu(II) complex in the N,N'-dipyridoxyl(1,2 diaminobenzene) was originally synthesized. Following, the breast cancer was transplanted with the TUBO cells in vivo. Then, treatment of the cancerous mice was done by low-frequency electromagnetic field and the Cu(II)-Schiff base complex. In this project, antiproliferative activity on breast cancer cells was tested by TUBO cells using MTT assay and apoptosis properties were studied by flow cytometry. The interaction between the Cu(II)-Schiff base complex and bovine serum albumin (BSA) was checked by fluorescence and UV-vis absorbance spectroscopy. Tumor tissue investigation demonstrated that the low-frequency electromagnetic field and Cu(II)-Schiff base complex induce apoptosis and inhibit tumor growth. MTT results unveiled a cytotoxic impact on breast cancer cells. Flow cytometry analysis demonstrates that the anticancer effect of Cu(II)-Schiff base complex on breast cancer cells (MCF7) was associated with the cell cycle arrest. The results of fluorescence spectra and UV-vis absorption spectra showed that the conformation of bovine serum albumin has been changed in the presence of Cu(II)-Schiff base complex. Cu(II)-Schiff base complex and low-frequency electromagnetic field have anticancer properties. The spectroscopy method indicates the binding between Cu(II)-Schiff base complex and BSA.

中文翻译:

光谱学研究Cu(II)席夫碱和低频电磁场的抗癌活性以及Cu(II)席夫碱与牛血清白蛋白之间的相互作用。

癌症是异常细胞增殖的结果,这导致异常肿块的形成。在这项研究中,我们旨在确定Cu(II)-席夫碱配合物和低频电磁场的抗癌性能,以及BSA与Cu(II)配合物之间的相互作用。首先,最初合成了N,N'-二吡啶氧基(1,2,二氨基苯)中Cu(II)配合物的席夫碱。随后,将乳腺癌与TUBO细胞一起体内移植。然后,通过低频电磁场和Cu(II)-席夫碱复合物对癌小鼠进行治疗。在该项目中,使用MTT法通过TUBO细胞测试了对乳腺癌细胞的抗增殖活性,并通过流式细胞术研究了其凋亡特性。Cu(II)-席夫碱复合物和牛血清白蛋白(BSA)之间的相互作用通过荧光和紫外可见吸收光谱法检查。肿瘤组织研究表明,低频电磁场和Cu(II)-席夫碱复合物可诱导细胞凋亡并抑制肿瘤生长。MTT结果揭示了对乳腺癌细胞的细胞毒性作用。流式细胞仪分析表明,Cu(II)-席夫碱复合物对乳腺癌细胞(MCF7)的抗癌作用与细胞周期停滞有关。荧光光谱和紫外可见吸收光谱的结果表明,在存在Cu(II)-席夫碱配合物的情况下,牛血清白蛋白的构象已经改变。Cu(II)-席夫碱配合物和低频电磁场具有抗癌性能。
更新日期:2020-03-03
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