Abstract

Cephalosporins, one of the classic β-lactam antibiotics, has been widely concerned by global population as the most commonly used broad-spectrum antibiotic. Serious health hazards are possible to be posed by quality control problems caused by its unstable structure, as well as food and environmental pollution introduced by improper use. Given the reasons above, the sensitive and valid methods for monitor and determination of cephalosporins in different matrices are urgently required. This review aims to provide a comprehensive overview on the current status of the pretreatment and analysis methods of cephalosporins in bulk drug, pharmaceutics, animal-derived foodstuffs including eggs, milk, meat, environment samples, and biological samples. Pretreatment methods including simple steps (protein precipitation, centrifugation, filtration), liquid-liquid extraction, solid phase extraction, QuECHERS, and detection methods covering LC, LC-MS/MS, voltammetric sensor, capillary electrophoresis spectroscopy, biological methods from January 2005 to October 2018 are updated, elaborated and compared here. Moreover, advanced materials and prospects for development are discussed.

1. Highlights

2. Determination of cephalosporins in different newly found matrix are represented.

3. Comparisons between different mass analyzers and progress in HRMS methods are in detailed.

4. Optimization of experimental conditions are discussed.

5. Newly emerged eco-friendly methods are introduced.

抽象的

头孢菌素，一种经典的β-内酰胺抗生素，已被全球人群广泛关注，认为是最常用的广谱抗生素。由于结构不稳定，质量控制问题以及使用不当引起的食品和环境污染，可能造成严重的健康危害。鉴于上述原因，迫切需要一种灵敏有效的方法来监测和测定不同基质中的头孢菌素。这篇综述旨在对散装药物，药物，包括鸡蛋，牛奶，肉类，环境样品和生物样品的动物源性食品中头孢菌素的预处理和分析方法的现状进行全面概述。预处理方法包括简单的步骤（蛋白质沉淀，离心，过滤），此处对2005年1月至2018年10月的液-液萃取，固相萃取，QuECHERS以及涵盖LC，LC-MS / MS，伏安传感器，毛细管电泳光谱法，生物学方法的检测方法进行了更新，阐述和比较。此外，还讨论了先进的材料和发展前景。

1. 强调

2. 代表了在新发现的不同基质中头孢菌素的测定。

3. 详细介绍了不同质量分析仪之间的比较以及HRMS方法的进展。

4. 讨论了实验条件的优化。

5. 介绍了新出现的环保方法。