Genipin is one of the major component in Gardenis fruit, which has long been used in the treatment of many chronic diseases, such as colitis. In the present study, we investigated the protective effects and mechanism of genipin on dextran sodium sulfate (DSS)-induced colitis in mice. Colitis was induced by giving 2.5% (wt/vol) DSS for 7 days. As the results show, DSS-induced body weight loss and colonic histological changes were inhibited by the treatment of genipin. DSS-induced MPO activity, MDA level, TNF-α, and IL-1β production in colonic tissues were also suppressed by genipin. To investigate the mechanism of genipin on DSS-induced colitis, the NF-κB and Nrf2 signaling pathways were detected. The results showed genipin significantly attenuated DSS-induced NF-κB activation and increased the expression of Nrf2 and HO-1 in a dose-dependent manner. The results of the present study indicated that genipin protected mice against colitis through inhibiting inflammatory and oxidative effects.

中文翻译：

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Genipin通过抑制炎症和氧化反应减轻硫酸葡聚糖钠诱导的结肠炎。

Genipin是Garden子果实的主要成分之一，长期以来一直用于治疗许多慢性疾病，例如结肠炎。在本研究中，我们研究了Genipin对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的保护作用及其机制。通过给予2.5％（wt / vol）DSS诱导结肠炎7天。结果表明，用genipin可以抑制DSS引起的体重减轻和结肠组织学改变。genipin还抑制了DSS诱导的结肠组织中MPO活性，MDA水平，TNF-α和IL-1β的产生。为了研究Genipin对DSS诱发的结肠炎的机制，检测了NF-κB和Nrf2信号通路。结果表明，Genipin剂量依赖性地显着减弱了DSS诱导的NF-κB活化，并增加了Nrf2和HO-1的表达。