BACKGROUND The pathophysiology of brain injury following aneurysmal subarachnoid haemorrhage (SAH) is associated with numerous mediators. The aim of the study is to analyse protein changes after SAH in cerebrospinal fluid (CSF) using mass spectrometry (MS). METHODS CSF samples were obtained from forty-four control subjects, seven good outcome and ten poor outcome SAH patients. CSF samples were collected at specific time intervals after SAH (days 1, 5 and 10). MALDI-TOF (Matrix Assisted Laser Desorption/Ionization Time-of-Flight) and ClinProTools software were utilised for MS, MS/MS (Mass Spectrometry) spectra collection and analysis. Selected masses were identified. The MALDI-TOF profiling experiments allowed for the targeted selection of potential markers in SAH. The study was performed in three steps by comparison of CSF samples: (1) from the control group and SAH patients (both good and poor outcome groups); (2) collected on days 1, 5 and 10 within the groups of poor SAH and good SAH patients, respectively; (3) from poor outcome SAH and good outcome patients at days 1, 5 and 10. RESULTS 15 new proteins whose CSF level is alternated by SAH presence, SAH treatment outcome and time passed since aneurysm rupture were identified. CONCLUSIONS We demonstrated new proteins which might play a role in different stages of subarachnoid haemorrhage and could be a new target for further investigation.

中文翻译：

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脑积水和蛛网膜下腔出血患者的脑脊液蛋白质组学。

背景动脉瘤性蛛网膜下腔出血 (SAH) 后脑损伤的病理生理学与许多介质有关。该研究的目的是使用质谱 (MS) 分析 SAH 后脑脊液 (CSF) 中的蛋白质变化。方法 CSF 样本取自 44 名对照受试者，7 名预后良好的 SAH 患者和 10 名预后不良的 SAH 患者。在 SAH 后的特定时间间隔（第 1、5 和 10 天）收集 CSF 样本。MALDI-TOF（基质辅助激光解吸/电离飞行时间）和 ClinProTools 软件用于 MS、MS/MS（质谱）光谱收集和分析。确定了选定的质量。MALDI-TOF 分析实验允许有针对性地选择 SAH 中的潜在标记。该研究通过比较 CSF 样本分三个步骤进行：(1) 来自对照组和 SAH 患者（预后良好组和不良组）；(2)分别于SAH差组和SAH好组内第1、5、10天采集；(3) 在第 1、5 和 10 天，来自预后不良的 SAH 和预后良好的患者。结果 确定了 15 种新蛋白，其 CSF 水平因 SAH 存在、SAH 治疗结果和自动脉瘤破裂以来经过的时间而变化。结论 我们证明了新的蛋白质可能在蛛网膜下腔出血的不同阶段发挥作用，并可能成为进一步研究的新目标。结果 确定了 15 种新蛋白质，其 CSF 水平因 SAH 存在、SAH 治疗结果和自动脉瘤破裂以来经过的时间而异。结论 我们证明了新的蛋白质可能在蛛网膜下腔出血的不同阶段发挥作用，并可能成为进一步研究的新目标。结果 确定了 15 种新蛋白质，其 CSF 水平因 SAH 存在、SAH 治疗结果和自动脉瘤破裂以来经过的时间而异。结论 我们证明了新的蛋白质可能在蛛网膜下腔出血的不同阶段发挥作用，并可能成为进一步研究的新目标。