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Visualization of hydrogen polysulfides in living cells and in vivo via a near-infrared fluorescent probe.
JBIC Journal of Biological Inorganic Chemistry ( IF 3 ) Pub Date : 2019-09-12 , DOI: 10.1007/s00775-019-01718-6
Xiaoqing Wang 1 , Qian Sun 1 , Liming Zhao 2 , Shuwen Gong 2 , Li Xu 1, 3
Affiliation  

Hydrogen polysulfides (H2Sn, n > 1), as the oxidized forms of H2S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H2Sn in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1, with "turn-on" response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H2Sn. The probe was successfully applied to the visualizing of endogenous H2Sn in living cells. Moreover, it can be used for near-infrared in vivo imaging of H2Sn in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H2Sn in living systems.

中文翻译:

通过近红外荧光探针观察活细胞和体内的多硫化氢。

作为硫化氢的氧化形式,多硫化氢(H2Sn,n> 1)由于近年来参与信号转导和细胞保护过程而受到越来越多的关注。有必要在生命系统中检测H2Sn,以研究其功能。在这项工作中,我们报告了基于BODIPY的近红外发射荧光探针NIR-PHS1,具有“开启”响应,快速响应速率(在10分钟内),出色的选择性和出色的灵敏度(检测限= 12 nM)响应朝向H2Sn。该探针已成功应用于可视化活细胞中的内源性H2Sn。此外,它可用于活小鼠中H2Sn的近红外体内成像。因此,NIR-PHS1可能是研究H2Sn在生命系统中的生物学作用的潜在成像工具。
更新日期:2019-11-01
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