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ErbB2 Copy Number Aberration in Canine Urothelial Carcinoma Detected by a Digital Polymerase Chain Reaction Assay.
Veterinary Pathology ( IF 2.4 ) Pub Date : 2019-10-22 , DOI: 10.1177/0300985819879445
Kosei Sakai 1 , Shingo Maeda 1 , Kohei Saeki 2 , Ryohei Yoshitake 2 , Yuko Goto-Koshino 3 , Takayuki Nakagawa 2 , Ryohei Nishimura 2 , Tomohiro Yonezawa 1 , Naoaki Matsuki 1
Affiliation  

Urothelial carcinoma (UC) is the most common tumor affecting the urinary bladder of dogs. Protein overexpression of ErbB2 (the canine homolog of HER2) has been observed in dogs with UC. However, no study regarding ErbB2 copy number aberration (CNA) is reported in dogs with UC. In this study, a digital PCR assay for detecting CNA of canine ErbB2 was developed. DNA samples were isolated from 83 formalin-fixed, paraffin-embedded urinary bladder tissues (36 UC, 8 polypoid cystitis, and 39 normal) and 94 urinary sediments (54 UC, 30 nonneoplastic, and 10 normal). The copy number of canine chromosome 8 (CFA8) was used as a control. In the urinary bladder tissues, ErbB2 CNA was detected in 12 of 36 (33%) UC, 2 of 8 (25%) polypoid cystitis, and 0 of 39 (0%) normal controls. In the urinary sediments, ErbB2 CNA was also detected in 19 of 54 (35%) UC; however, no ErbB2 CNA was detected in nonneoplastic diseases or normal controls. The sensitivity and specificity of ErbB2 CNA in urinary sediment for the detection of UC were 35% and 100%, respectively. There was a positive correlation between the copy number ratios of ErbB2 to CFA8 in the urinary bladder tissues and urinary sediments. Our findings indicate that the digital PCR assay of urinary sediments may be a useful, noninvasive method for detecting ErbB2 CNA in dogs with UC.

中文翻译:

通过数字聚合酶链反应测定法检测犬尿道上皮癌的ErbB2拷贝数异常。

尿路上皮癌(UC)是影响犬膀胱的最常见肿瘤。在患有UC的犬中已经观察到ErbB2蛋白的过量表达(HER2的犬同源物)。然而,没有关于患有UC的犬中关于ErbB2拷贝数畸变(CNA)的研究的报道。在这项研究中,开发了一种用于检测犬ErbB2 CNA的数字PCR检测方法。从83个福尔马林固定,石蜡包埋的膀胱组织(36 UC,8个息肉样膀胱炎和39个正常)和94个尿沉渣(54 UC,30个非肿瘤性和10个正常)中分离出DNA样品。用犬8号染色体(CFA8)的拷贝数作为对照。在膀胱组织中,在36例UC中有12例(33%),8例(25%)息肉样膀胱炎中有2例和39例(0%)正常对照中有0例检测到ErbB2 CNA。在尿沉渣中,在54个(35%)UC中有19个还检测到ErbB2 CNA。但是,在非肿瘤性疾病或正常对照中未检测到ErbB2 CNA。ErbB2 CNA在尿沉渣中检测UC的敏感性和特异性分别为35%和100%。膀胱组织和尿沉渣中ErbB2与CFA8的拷贝数比之间呈正相关。我们的发现表明,尿沉渣的数字PCR检测可能是一种有用的,无创的方法,用于检测UC犬的ErbB2 CNA。
更新日期:2019-11-01
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