Abstract 6-Tuliposides A (6-PosA) and B (6-PosB) are major defensive secondary metabolites in tulip cultivars (Tulipa gesneriana), having an acyl group at the C-6 position of d-glucose. Although some wild tulip species produce 1,6-diacyl-glucose type of Pos (PosD and PosF), as well as 6-PosA/B, they have not yet been isolated from tulip cultivars. Here, aiming at verifying the presence of PosD and PosF in tulip cultivars, tissue extracts of 25 cultivars were analyzed by high-performance liquid chromatography (HPLC). Although no HPLC peaks for PosD nor PosF were detected in most cultivars, we found two cultivars giving a minute HPLC peak for PosD and the other two cultivars giving that for PosF. PosD and PosF were then purified from petals of cultivar ‘Orca’ and from pistils of cultivar ‘Murasakizuisho’, respectively, and their identities were verified by spectroscopic analyses. This is the first report that substantiates the presence of 1,6-diacyl-glucose type of Pos in tulip cultivars.

中文翻译：

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郁金香品种郁金香苷D和F的分离鉴定

摘要 6-郁金香苷 A (6-PosA) 和 B (6-PosB) 是郁金香品种 (Tulipa gesneriana) 中的主要防御性次生代谢产物，在 d-葡萄糖的 C-6 位具有一个酰基。尽管一些野生郁金香物种产生 1,6-二酰基葡萄糖型 Pos（PosD 和 PosF）以及 6-PosA/B，但尚未从郁金香品种中分离出来。在这里，为了验证郁金香品种中 PosD 和 PosF 的存在，通过高效液相色谱 (HPLC) 分析了 25 个品种的组织提取物。尽管在大多数品种中没有检测到 PosD 和 PosF 的 HPLC 峰，但我们发现两个品种为 PosD 提供了一个微小的 HPLC 峰，而其他两个品种为 PosF 提供了那个。PosD 和 PosF 然后分别从栽培品种 'Orca' 的花瓣和栽培品种 'Murasakizuisho' 的雌蕊中纯化出来，并通过光谱分析验证了它们的身份。这是第一份证实郁金香品种中存在 1,6-二酰基-葡萄糖型 Pos 的报告。