Naturally-derived polysaccharides, such as alginate and chitosan, can be assembled to form nanocarriers for the delivery of therapeutic agents. Here we exploit the electrostatic complexation of alginate/chitosan in a water-in-oil (w/o) emulsion process to produce doxorubicin (DOX)-loaded nanoparticles (~80 nm) with exceptional spherical morphology and uniformity. This robust synthetic route utilizes an aqueous phase dispersed in a cyclohexane/dodecylamine organic phase and is capable of encapsulating DOX in the nanoparticle solution. The uptake and efficacy of this novel formulation was evaluated in a murine breast cancer cell line, 4T1, with comparable 72 h IC₅₀ values of the nanoparticle solution (0.15 μg/mL) and free DOX (0.13 μg/mL). Overall, the favorable performance, physiochemical properties, and their facile production support these nanocarriers as promising platform for the delivery of aqueous soluble drugs.

天然来源的多糖，例如藻酸盐和壳聚糖，可以被组装形成用于递送治疗剂的纳米载体。在这里，我们利用油包水（w / o）乳化工艺中藻酸盐/壳聚糖的静电络合来生产负载阿霉素（DOX）的纳米颗粒（〜80 nm），并具有出色的球形形态和均匀性。这种稳健的合成途径利用了分散在环己烷/十二烷基胺有机相中的水相，并且能够将DOX包封在纳米颗粒溶液中。在鼠乳腺癌细胞系4T1中评估了这种新型制剂的摄取和功效，可比较72 h IC ₅₀纳米颗粒溶液（0.15μg/ mL）和游离DOX（0.13μg/ mL）的数值。总体而言，良好的性能，理化性质及其简便的生产方法支持这些纳米载体成为水溶性药物输送的有前途的平台。