Male karyotype and meiosis in four true bug species belonging to the families Reduviidae, Nabidae, and Miridae (Cimicomorpha) were studied for the first time using Giemsa staining and FISH with 18S ribosomal DNA and telomeric (TTAGG)n probes. We found that Rhynocoris punctiventris (Herrich-Schäffer, 1846) and R. iracundus (Poda, 1761) (Reduviidae: Harpactorinae) had 2n = 28 (24 + X1X2X3Y), whereas Nabis sareptanus Dohrn, 1862 (Nabidae) and Horistus orientalis (Gmelin, 1790) (Miridae) had 2n = 34 (32 + XY) and 2n = 32 (30 + XY), respectively. FISH for 18S rDNA revealed hybridization signals on a sex chromosome, the X or the Y, in H. orientalis, on both X and Y chromosomes in N. sareptanus, and on two of the four sex chromosomes, Y and one of the Xs, in both species of Rhynocoris Hahn, 1834. The results of FISH with telomeric probes support with confidence the absence of the "insect" telomere motif (TTAGG)n in the families Nabidae and Miridae and its presence in both species of genus Rhynocoris of the Reduviidae, considered as a basal family of Cimicomorpha. Increasing evidence reinforces the hypothesis of the loss of the canonical "insect" telomere motif (TTAGG)n by at least four cimicomorphan families, Nabidae, Miridae, Tingidae, and Cimicidae, for which data are currently available.

中文翻译：

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端粒基序 (TTAGG) n 在 Reduviidae 科中存在而在 Nabidae 和 Miridae 科（半翅目，Cimicomorpha）中不存在的新证据。

首次使用吉姆萨染色和 FISH 与 18S 核糖体 DNA 和端粒 (TTAG)n 探针研究了属于 Reduviidae、Nabidae 和 Miridae (Cimicomorpha) 的四种真正昆虫物种的雄性核型和减数分裂。我们发现 Rhynocoris punctiventris (Herrich-Schäffer, 1846) 和 R. iracundus (Poda, 1761) (Reduviidae: Harpactorinae) 有 2n = 28 (24 + X1X2X3Y)，而 Nabis sareptanus Dohrn, 1862 (Nabid orientae , 1790) (Miridae) 分别有 2n = 34 (32 + XY) 和 2n = 32 (30 + XY)。18S rDNA 的 FISH 揭示了在 H. orientalis 中的一条性染色体 X 或 Y 上，在 N. sareptanus 中的 X 和 Y 染色体上，以及在四个性染色体中的两个，Y 和一个 X 上的杂交信号，在 Rhynocoris Hahn 的两个物种中，1834。FISH 与端粒探针的结果自信地支持 Nabidae 和 Miridae 家族中不存在“昆虫”端粒基序 (TTGG)n，并且它在 Reduviidae 的 Rhynocoris 属的两个物种中都存在，被认为是 Cimicomorpha 的一个基础家族。越来越多的证据加强了至少四个 cimicomorphan 科，Nabidae，Miridae，Tingidae 和 Cimicidae 失去了规范“昆虫”端粒基序 (TTAG)n 的假设，目前有数据可用。