Abstract Introduction Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in veterinary medicine. They are used in pain control and in anti-inflammatory and antipyretic therapies. Some NSAIDs, e.g piroxicam, also have a documented anticancer effect. The objective of this study was to evaluate which of the commonly used NSAIDs (etodolac, flunixin, tolfenamic acid, carprofen, and ketoprofen) are cytotoxic to the D-17 cell line of canine osteosarcoma. Material and Methods The viability of the cells was evaluated using the MTT assay. Four independent repetitions were performed and the results are given as the average of these values; EC50 values (half maximal effective concentration) were also calculated. Results The analysis of results showed that carprofen and tolfenamic acid displayed the highest cytotoxicity. Other drugs either did not provide such effects or they were very poor. For carprofen, it was possible to determine an EC50 which fell within the limits of concentrations obtainable in canine serum after the administration of routinely used doses. Conclusion The results are promising but further studies should be conducted to confirm them, since this study is only preliminary. The possibility of introducing carprofen and tolfenamic acid into the routine treatment of osteosarcoma in dogs should be considered.

中文翻译：

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选定的非甾体抗炎药对 D-17 系犬骨肉瘤细胞活力的影响：体外研究

摘要 介绍 非甾体抗炎药（NSAIDs）广泛用于兽医学。它们用于疼痛控制以及抗炎和解热疗法。一些非甾体抗炎药，例如吡罗昔康，也有记载的抗癌作用。本研究的目的是评估哪些常用的非甾体抗炎药（依托度酸、氟尼辛、托芬那酸、卡洛芬和酮洛芬）对犬骨肉瘤的 D-17 细胞系具有细胞毒性。材料和方法使用MTT测定评估细胞的活力。进行了四次独立重复，结果以这些值的平均值给出；还计算了 EC50 值（最大有效浓度的一半）。结果结果分析表明，卡洛芬和托芬那酸的细胞毒性最高。其他药物要么没有提供这种效果，要么效果很差。对于卡洛芬，可以确定 EC50 在常规使用剂量给药后在犬血清中可获得的浓度范围内。结论 结果是有希望的，但应该进行进一步的研究来证实它们，因为这项研究只是初步的。应考虑将卡洛芬和托芬那酸引入犬骨肉瘤常规治疗的可能性。因为这项研究只是初步的。应考虑将卡洛芬和托芬那酸引入犬骨肉瘤常规治疗的可能性。因为这项研究只是初步的。应考虑将卡洛芬和托芬那酸引入犬骨肉瘤常规治疗的可能性。