Abstract Introduction Comparative analysis of the karyotype structure was made in two hedgehog species: the northern white-breasted hedgehog (Erinaceus roumanicus) and the African pygmy hedgehog (Atelerix albiventris). Material and Methods The cytogenetic analysis used differential staining techniques (DAPI, Ag-NOR, and C-banding/DAPI) and sequential QFQ/FISH banding with NOR20 and TEL20 probes which showed 45S rDNA and (TTAGGG)n repeat sequences, respectively, on hedgehog chromosomes. Results It was confirmed that the somatic cells of the hedgehogs have a constant number of chromosomes (2n = 48,XY). Differences were observed in the NOR number between the species. NORs were identified on three autosome pairs in the northern white-breasted hedgehog and on only two pairs in the African pygmy hedgehog. Chromosome analysis by C-banding/DAPI showed large segments of heterochromatin rich in A-T pairs on three autosome pairs in both the northern white-breasted and African pygmy hedgehogs. The heterochromatin segments encompassed large fragments of the longer arm of chromosome pairs 13, 14 and 20. The (TTAGGG)n repeat sequences on the hedgehog chromosomes were only observed in the terminal position of all the chromosomes in both species. Conclusion Our observations provide new information on the level of diversity within the Erinaceidae family.

中文翻译：

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鸢尾科特定物种的细胞遗传学核型分析

摘要 介绍 对两种刺猬的核型结构进行了比较分析：北方白胸刺猬（Erinaceus roumanicus）和非洲侏儒刺猬（Atelerix albiventris）。材料和方法 细胞遗传学分析使用差异染色技术（DAPI、Ag-NOR 和 C 带/DAPI）和顺序 QFQ/FISH 带，用 NOR20 和 TEL20 探针分别显示 45S rDNA 和 (TTAGGG)n 重复序列，刺猬染色体。结果证实刺猬的体细胞染色体数目恒定（2n=48，XY）。在物种之间观察到NOR数的差异。在北部白胸刺猬的三对常染色体和非洲侏儒刺猬的两对常染色体上发现了 NOR。通过 C 带/DAPI 进行的染色体分析显示，在北部白胸刺猬和非洲侏儒刺猬的三对常染色体上，大量异染色质富含 AT 对。异染色质片段包含染色体对13、14和20的较长臂的大片段。刺猬染色体上的(TTAGGG)n重复序列仅在两个物种的所有染色体的末端位置观察到。结论 我们的观察结果提供了关于鸢尾科内多样性水平的新信息。刺猬染色体上的(TTAGGG)n重复序列仅在两个物种的所有染色体的末端位置观察到。结论 我们的观察结果提供了关于鸢尾科内多样性水平的新信息。刺猬染色体上的(TTAGGG)n重复序列仅在两个物种的所有染色体的末端位置观察到。结论 我们的观察结果提供了关于鸢尾科内多样性水平的新信息。