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Phenotypic diversity and potential virulence factors of the Shewanella putrefaciens group isolated from freshwater fish
Journal of Veterinary Research ( IF 1.8 ) Pub Date : 2019-09-13 , DOI: 10.2478/jvetres-2019-0046
Ewa Paździor 1 , Agnieszka Pękala-Safińska 1 , Dariusz Wasyl 2
Affiliation  

Abstract Introduction The Shewanella putrefaciens group are ubiquitous microorganisms recently isolated from different freshwater fish species and causing serious health disorders. The purpose of the study was to characterise isolates of the S. putrefaciens group with special emphasis on elucidating serological diversity and determining putative virulence factors. Material and Methods Isolates collected from freshwater fish (n = 44) and reference strains were used. The identification of bacteria was carried out using biochemical kits and 16S rRNA sequencing. Polyclonal antibodies were prepared against the S. putrefaciens group. The bacterium’s susceptibility to antimicrobial agents, its enzymatic properties, and its adhesion ability to fish cell lines were also tested. Finally, selected isolates were used in challenge experiments in common carp and rainbow trout. Results Excluding six isolates undeterminable for species, the bacteria were classified to three species: S. putrefaciens, S. xiamenensis, and S. oneidensis, and showed some phenotypic diversity. Fourteen serological variants of the S. putrefaciens group were determined with the newly developed serotyping scheme. Conclusion Serodiversity may play an important role in the virulence of particular isolates. Further, S. putrefaciens group members adhere to epithelial cells and produce enzymes which may contribute to their virulence. Challenge tests confirmed the pathogenicity of the S. putrefaciens group for fish.

中文翻译:

从淡水鱼中分离的腐臭希瓦氏菌群的表型多样性和潜在毒力因子

摘要 简介 腐烂希瓦氏菌群是最近从不同淡水鱼类中分离出来的普遍存在的微生物,可导致严重的健康问题。该研究的目的是表征腐烂链球菌组的分离株,特别强调阐明血清学多样性和确定假定的毒力因子。材料和方法 使用从淡水鱼 (n = 44) 和参考菌株中收集的分离株。使用生化试剂盒和16S rRNA测序进行细菌鉴定。制备了针对腐败链球菌组的多克隆抗体。还测试了该细菌对抗菌剂的敏感性、酶的特性以及对鱼细胞系的粘附能力。最后,选定的分离株用于鲤鱼和虹鳟鱼的挑战实验。结果除无法确定种属的6个分离株外,细菌分为三个种:腐烂链霉菌、厦门链霉菌和奥尼链链霉菌,并表现出一定的表型多样性。用新开发的血清分型方案确定了腐败链球菌组的 14 种血清学变体。结论 血清多样性可能在特定分离株的毒力中起重要作用。此外,腐烂链球菌组成员粘附上皮细胞并产生可能有助于其毒力的酶。挑战试验证实了腐败链球菌对鱼类的致病性。和 S. oneidensis,并显示出一些表型多样性。用新开发的血清分型方案确定了腐败链球菌组的 14 种血清学变体。结论 血清多样性可能在特定分离株的毒力中起重要作用。此外,腐烂链球菌组成员粘附上皮细胞并产生可能有助于其毒力的酶。挑战试验证实了腐败链球菌对鱼类的致病性。和 S. oneidensis,并显示出一些表型多样性。用新开发的血清分型方案确定了腐败链球菌组的 14 种血清学变体。结论 血清多样性可能在特定分离株的毒力中起重要作用。此外,腐烂链球菌组成员粘附上皮细胞并产生可能有助于其毒力的酶。挑战试验证实了腐败链球菌对鱼类的致病性。腐烂菌群成员粘附上皮细胞并产生可能有助于其毒力的酶。挑战试验证实了腐败链球菌对鱼类的致病性。腐烂菌群成员粘附上皮细胞并产生可能有助于其毒力的酶。挑战试验证实了腐败链球菌对鱼类的致病性。
更新日期:2019-09-13
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