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Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA), SOD, CAT and GPx in Freund's adjuvant inflammation-induced male rats.
Journal of Inflammation Research ( IF 4.5 ) Pub Date : 2019-09-03 , DOI: 10.2147/jir.s212577 M H Meshkibaf 1 , M Maleknia 1 , S Noroozi 1
Journal of Inflammation Research ( IF 4.5 ) Pub Date : 2019-09-03 , DOI: 10.2147/jir.s212577 M H Meshkibaf 1 , M Maleknia 1 , S Noroozi 1
Affiliation
Objective: Curcumin is the well-known compound which is extracted from turmeric powder, the dried rhizome of the Curcuma longa Linn. This have been used for the treatment of various disorders including inflammation. In this study we have analyzed the effect of curcumin on arthritis induced by adjuvant in rats, considering changes in methionine sulfoxide reductase A (MSRA) expression and antioxidant enzymes levels.
Methods: Five groups of adult male Wistar rats (n=10), were randomly selected as control, placebo, experimental 1, 2 and 3. The induction of arthritis was carried out by injection of 0.1 ml adjuvant in plantar region. The first experimental group received no curcumin treatment, whereas the experimental two and three received curcumin (1 and 2 g/kg daily) respectively, for fourteen days. MSRA gene expression was assessed by real-time PCR and protein levels of MSRA, SOD, CAT and GPx were analyzed via ELISA method.
Results: The results showed no significant weight changes among the groups during the experimental period and the paw swelling caused by adjuvant was recovered within fourteen days of treatment with curcumin. However, the levels of enzymes such as superoxide dismutase, catalase and glutathione peroxidase were increased by a dose dependent manner. These results also illustrated that the gene expression and protein level of MSRA in groups treated with curcumin increased significantly (p≤0.05).
Conclusion: We concluded that the curcumin can be used against inflammation. The increasing level of MSRA can be due to the antioxidant effect of curcumin. The enzymatic level changes (MSRA, SOD, CAT and GPx) may interfere with the aging process and delay it.
Keywords: curcumin, anti-inflammation, MSRA
中文翻译：
姜黄素对弗氏佐剂炎症诱导雄性大鼠甲硫氨酸亚砜还原酶 A (MSRA)、SOD、CAT 和 GPx 基因表达和蛋白水平的影响。
目的：姜黄素是众所周知的化合物，是从姜黄的干燥根茎姜黄粉中提取的。这已被用于治疗包括炎症在内的各种疾病。在这项研究中，我们分析了姜黄素对大鼠佐剂诱导的关节炎的影响，考虑了甲硫氨酸亚砜还原酶 A (MSRA) 表达和抗氧化酶水平的变化。
方法：5组成年雄性Wistar大鼠（n=10），随机选择对照组、安慰剂组、实验组1、2和3。通过足底注射佐剂0.1ml进行关节炎的诱导。第一个实验组没有接受姜黄素治疗，而实验二和三分别接受姜黄素（每天 1 和 2 g/kg），持续 14 天。MSRA基因表达通过实时PCR评估，MSRA、SOD、CAT和GPx蛋白水平通过ELISA方法分析。
结果：结果显示，在实验期间各组之间的体重没有显着变化，并且由佐剂引起的爪肿胀在姜黄素治疗的十四天内恢复。然而，酶如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平以剂量依赖性方式增加。这些结果也说明姜黄素治疗组MSRA基因表达和蛋白水平显着升高（p≤0.05）。
结论：我们得出结论，姜黄素可用于抗炎。MSRA 水平的增加可能是由于姜黄素的抗氧化作用。酶水平变化（MSRA、SOD、CAT 和 GPx）可能会干扰衰老过程并延缓衰老。
关键词：姜黄素，抗炎，MSRA
更新日期：2019-09-03
Methods: Five groups of adult male Wistar rats (n=10), were randomly selected as control, placebo, experimental 1, 2 and 3. The induction of arthritis was carried out by injection of 0.1 ml adjuvant in plantar region. The first experimental group received no curcumin treatment, whereas the experimental two and three received curcumin (1 and 2 g/kg daily) respectively, for fourteen days. MSRA gene expression was assessed by real-time PCR and protein levels of MSRA, SOD, CAT and GPx were analyzed via ELISA method.
Results: The results showed no significant weight changes among the groups during the experimental period and the paw swelling caused by adjuvant was recovered within fourteen days of treatment with curcumin. However, the levels of enzymes such as superoxide dismutase, catalase and glutathione peroxidase were increased by a dose dependent manner. These results also illustrated that the gene expression and protein level of MSRA in groups treated with curcumin increased significantly (p≤0.05).
Conclusion: We concluded that the curcumin can be used against inflammation. The increasing level of MSRA can be due to the antioxidant effect of curcumin. The enzymatic level changes (MSRA, SOD, CAT and GPx) may interfere with the aging process and delay it.
Keywords: curcumin, anti-inflammation, MSRA
中文翻译：
姜黄素对弗氏佐剂炎症诱导雄性大鼠甲硫氨酸亚砜还原酶 A (MSRA)、SOD、CAT 和 GPx 基因表达和蛋白水平的影响。
目的：姜黄素是众所周知的化合物，是从姜黄的干燥根茎姜黄粉中提取的。这已被用于治疗包括炎症在内的各种疾病。在这项研究中，我们分析了姜黄素对大鼠佐剂诱导的关节炎的影响，考虑了甲硫氨酸亚砜还原酶 A (MSRA) 表达和抗氧化酶水平的变化。
方法：5组成年雄性Wistar大鼠（n=10），随机选择对照组、安慰剂组、实验组1、2和3。通过足底注射佐剂0.1ml进行关节炎的诱导。第一个实验组没有接受姜黄素治疗，而实验二和三分别接受姜黄素（每天 1 和 2 g/kg），持续 14 天。MSRA基因表达通过实时PCR评估，MSRA、SOD、CAT和GPx蛋白水平通过ELISA方法分析。
结果：结果显示，在实验期间各组之间的体重没有显着变化，并且由佐剂引起的爪肿胀在姜黄素治疗的十四天内恢复。然而，酶如超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的水平以剂量依赖性方式增加。这些结果也说明姜黄素治疗组MSRA基因表达和蛋白水平显着升高（p≤0.05）。
结论：我们得出结论，姜黄素可用于抗炎。MSRA 水平的增加可能是由于姜黄素的抗氧化作用。酶水平变化（MSRA、SOD、CAT 和 GPx）可能会干扰衰老过程并延缓衰老。
关键词：姜黄素，抗炎，MSRA
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