Sambucus nigra L. (Elderberry) is widely used as a dietary supplement in functional food and possesses many pharmacological activities to prevent ailments, such as the colds and fever, diabetes and cancer. However, research on its skin anti-aging effect is still limited. Here, we evaluated the recovery effects of elderberry extract (EB) in UVB-irradiated human skin keratinocytes (HaCaTs) and investigated whether EB represents a potential therapeutic agent against skin photoaging and inflammation. In this study, EB showed good efficiency on scavenging free radicals and dose-dependently reduced reactive oxygen species (ROS) generation. EB notably decreased UVB-induced matrix metalloproteinase-1 (MMP-1) expression and inflammatory cytokine secretion through the inhibition of mitogen-activated protein kinases/activator protein 1 (MAPK/AP-1) and nuclear factor-κB (NF-κB) signaling pathways, blocking extracellular matrix (ECM) degradation and inflammation in UVB-irradiated HaCaTs. In addition, EB improved nuclear factor E2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling to increase oxidative defense capacity, and enhanced transforming growth factor beta (TGF-β) signaling activation to promote procollagen type I synthesis, relieving UVB-induced skin cell damage. These results indicated that EB has the potential to ameliorate UVB-induced skin photoaging and inflammation.

中文翻译：

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黑色接骨木改善人皮肤角质形成细胞中UVB诱导的光老化和炎症反应。

接骨木（接骨木浆果）被广泛用作功能性食品中的膳食补充剂，并具有许多药理活性，可预防疾病，如感冒，发烧，糖尿病和癌症。然而，对其皮肤抗衰老作用的研究仍然有限。在这里，我们评估了接骨木提取物（EB）在UVB照射的人皮肤角质形成细胞（HaCaTs）中的恢复效果，并研究了EB是否代表针对皮肤光老化和炎症的潜在治疗剂。在这项研究中，EB显示出清除自由基和剂量依赖性减少活性氧（ROS）生成的良好效率。EB通过抑制有丝分裂原激活的蛋白激酶/激活蛋白1（MAPK / AP-1）和核因子-κB（NF-κB）明显降低了UVB诱导的基质金属蛋白酶1（MMP-1）的表达和炎性细胞因子的分泌。信号传导途径，阻断UVB照射的HaCaT中的细胞外基质（ECM）降解和炎症。此外，EB改善了核因子E2相关因子2 /血红素加氧酶-1（Nrf2 / HO-1）信号传导，从而增加了氧化防御能力，并增强了转化生长因子β（TGF-β）信号传导，从而促进了I型胶原原的合成。 ，缓解UVB引起的皮肤细胞损伤。这些结果表明，EB具有改善UVB诱导的皮肤光老化和炎症的潜力。阻断UVB照射的HaCaT中的细胞外基质（ECM）降解和炎症。此外，EB改善了核因子E2相关因子2 /血红素加氧酶-1（Nrf2 / HO-1）信号传导，从而增加了氧化防御能力，并增强了转化生长因子β（TGF-β）信号传导，从而促进了I型胶原原的合成。 ，缓解UVB引起的皮肤细胞损伤。这些结果表明，EB具有改善UVB诱导的皮肤光老化和炎症的潜力。阻断UVB照射的HaCaT中的细胞外基质（ECM）降解和炎症。此外，EB改善了核因子E2相关因子2 /血红素加氧酶-1（Nrf2 / HO-1）信号传导，从而增加了氧化防御能力，并增强了转化生长因子β（TGF-β）信号传导，从而促进了I型胶原原的合成。 ，缓解UVB引起的皮肤细胞损伤。这些结果表明，EB具有改善UVB诱导的皮肤光老化和炎症的潜力。