High temperature and salinity stress are major factors limiting the growth and productivity of rice crop on a global scale. It is therefore an essential prerequisite to understand the molecular genetic regulation of plant responses to dual stresses. MicroRNAs (miRs) are recognized as key controllers of gene expression which act mainly at the post-transcriptional level to regulate various aspects of plant development. The present study attempts to investigate the miR circuits that are modulated in response to high temperature and salinity stress in rice. To gain insights into the pathway, preliminary miR profiles were generated using the next-generation sequencing (NGS) datasets. The identified molecules were filtered on the basis of fold differential regulation under high temperature, and time kinetics of their expression under the two individual stresses was followed to capture the regulatory windows. The analysis revealed the involvement of common miR regulatory nodes in response to two different abiotic stresses, thereby broadening our perspective about the stress-mediated regulatory mechanisms operative in rice.

中文翻译：

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在高温和盐胁迫下鉴定水稻Pusa Basmati-1的microRNA靶模块。

高温和盐分胁迫是在全球范围内限制水稻作物生长和生产力的主要因素。因此，了解植物对双重胁迫反应的分子遗传调控是必不可少的前提。MicroRNA（miR）被认为是基因表达的关键控制器，主要在转录后水平上调节植物发育的各个方面。本研究试图研究在水稻中响应高温和盐分胁迫而被调节的miR回路。为了深入了解该途径，使用下一代测序（NGS）数据集生成了初步的miR图谱。在高温下根据倍数差异调节对鉴定出的分子进行过滤，然后在两个单独的应力下观察它们的表达的时间动力学，以捕获调节窗口。分析揭示了常见的miR调控节点参与了对两种不同的非生物胁迫的响应，从而拓宽了我们对水稻中介导的调控机制的认识。