Single-cell RNA sequencing (scRNA-seq) technologies are increasingly being applied to reveal cellular heterogeneity in kidney development and disease. In just the last year, multiple scRNA-seq datasets have been generated from kidney organoids, developing mouse and human kidney, adult kidney, and kidney cancer. The data generated enables a much deeper understanding of biological processes within and between cells. It has also elucidated unforeseen cell lineage relationships, defined the presence of off-target cell types in kidney organoids, and revealed a diverse inflammatory response in a human kidney allograft undergoing rejection. This review summarizes the recent rapid progress in scRNA-seq of the kidney and outlines future directions for single-cell technologies as applied to the kidney.

中文翻译：

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肾脏和类器官单细胞转录组学：开始的结束。

单细胞 RNA 测序 (scRNA-seq) 技术越来越多地用于揭示肾脏发育和疾病中的细胞异质性。就在去年，多个 scRNA-seq 数据集从肾脏类器官生成，发展为小鼠和人类肾脏、成人肾脏和肾癌。生成的数据有助于更深入地了解细胞内和细胞间的生物过程。它还阐明了无法预料的细胞谱系关系，确定了肾脏类器官中脱靶细胞类型的存在，并揭示了正在接受排斥反应的人类肾脏同种异体移植物中的多种炎症反应。这篇综述总结了肾脏 scRNA-seq 的近期快速进展，并概述了单细胞技术应用于肾脏的未来方向。