The need for direct biomaterial-based delivery of growth factors to wound surfaces to aid in wound healing emphasizes the importance of interfacial interactions between the biomaterial and the wound surface. These interactions include the spatial localization of growth factor, the surface intensity of growth factor in contact with the wound, and the release profile of growth factor to the wound surface. The authors report the use of time-of-flight secondary ion mass spectrometry to determine the relationship between biomaterial surface chemistry and the spatial localization of growth factor. They have implemented a novel application of total internal reflectance fluorescence (TIRF) microscopy to measure the surface intensity and release of growth factor in contact with a glass substrate that has been used to model a wound surface. Detailed information regarding TIRF experiments has been included to aid in future studies regarding the biomaterial delivery to interfaces. The authors have evaluated the effects of (hydroxyethyl)methacrylate (HEMA) homopolymer, 5.89% methyl methacrylate/HEMA, and 5.89% methacrylic acid/HEMA surface chemistry on the spatial localization of AlexaFluor 488-labeled keratinocyte growth factor (AF488-KGF), AF488-KGF surface intensity at the copolymer surface, and release to a glass substrate. KGF is known to promote re-epithelialization in wound healing. The results show that the two copolymers allow for increased surface coverage, surface intensity, and release of AF488-KGF in comparison to the homopolymer. It is likely that differences in these three aspects could have a profound effect on the wound healing response.

中文翻译：

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ToF-SIMS和TIRF显微镜研究了HEMA共聚物表面化学对空间定位，表面强度和荧光标记的角质形成细胞生长因子释放的影响。

需要将基于生物材料的生长因子直接递送至伤口表面以帮助伤口愈合，这强调了生物材料与伤口表面之间的界面相互作用的重要性。这些相互作用包括生长因子的空间定位，与伤口接触的生长因子的表面强度以及生长因子向伤口表面的释放曲线。作者报告了飞行时间二次离子质谱仪的使用，以确定生物材料表面化学与生长因子的空间定位之间的关系。他们已经实现了全内反射荧光（TIRF）显微镜的新应用，以测量与玻璃基板接触的表面强度和生长因子的释放，该玻璃基板已用于模拟伤口表面。已包含有关TIRF实验的详细信息，以帮助将来进行有关将生物材料输送至界面的研究。作者评估了（羟乙基）甲基丙烯酸酯（HEMA）均聚物，5.89％甲基丙烯酸甲酯/ HEMA和5.89％甲基丙烯酸/ HEMA表面化学对AlexaFluor 488标记的角质形成细胞生长因子（AF488-KGF）的空间定位的影响， AF488-KGF在共聚物表面的表面强度高，并释放到玻璃基板上。已知KGF可促进伤口愈合中的上皮再生。结果表明，与均聚物相比，这两种共聚物可以提高表面覆盖率，表面强度和AF488-KGF的释放。这三个方面的差异可能会对伤口愈合反应产生深远影响。