Polyelectrolyte multilayers (PEMs) consisting of hyaluronic acid (HA) and chitosan (Ch) are extensively studied for biomedical applications and suppress bacterial and protein attachment. Here, we prepared and tested HA/Ch PEMs as marine fouling-release coatings. PEMs were constructed by layer-by-layer assembly using spin coating. The multilayers were crosslinked for enhanced stability in the sea water environment by chemical and thermal treatment. Protein-repelling properties of the crosslinked multilayers were investigated by surface plasmon resonance spectroscopy. The marine antifouling and fouling-release properties were tested against the settlement of zoospores of the green alga Ulva linza and the subsequent development and removal of sporelings. With spin coating and thermal crosslinking, a thick yet homogeneous coating was obtained with antifouling properties against marine algal zoospores indicating the potential of these compounds for application in protective coatings.

中文翻译：

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逐层构造透明质酸/壳聚糖多层作为防污和防污涂料。

由透明质酸（HA）和壳聚糖（Ch）组成的聚电解质多层（PEM）已广泛研究用于生物医学应用并抑制细菌和蛋白质的附着。在这里，我们准备并测试了HA / Ch PEM作为船用防污涂料。使用旋涂通过逐层组装来构造PEM。通过化学和热处理使多层交联以增强在海水环境中的稳定性。通过表面等离振子共振光谱研究了交联的多层的蛋白质排斥特性。测试了海洋防污垢和污垢释放性能，以防绿藻Ulva linza游动孢子的沉降以及随后的孢子形成和去除。通过旋涂和热交联，