Infection due to Entamoeba spp. is known to cause serious disease in primates (Entamoeba histolytica) and snakes (Entamoeba invadens), but there are no detailed descriptions of the pathology associated with Entamoeba spp. infection in amphibians. In 2014, an outbreak of entamoebiasis associated with a novel species of Entamoeba induced clinical illness and poor body condition in free-ranging cane toads in Australia's Northern Territory. Here, we describe the gross pathology, histology, and clinical pathology linked to the outbreak. The study compared 25 toads with invasive entamoebiasis, defined as histologically visible amoebas within tissue, and 12 toads without invasive entamoebiasis. Grossly, affected toads had mild to marked congestion of colonic serosal vasculature, with variable thickening of the intestinal wall and serosanguineous to hemorrhagic colonic content. Histologically, invasive entamoebiasis manifested primarily as moderate to severe, variably hyperplastic to ulcerative colitis. The small intestine was affected in 10 of 25 toads, and 5 of 25 toads also had gastric lesions. Amoebas consistent in morphology with Entamoeba sp. were commonly intermingled with mucosal epithelium, frequently along the basement membrane, with deeper invasion into the superficial lamina propria in only 5 toads. Toads with invasive entamoebiasis had neutrophilia, monocytosis, and lymphopenia, and thus elevated neutrophil to lymphocyte ratios, suggestive of an inflammatory and/or stress leukogram.

中文翻译：

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与热带澳大利亚野生甘蔗蟾蜍（Rhinella marina）中肠轮虫病暴发相关的病理学。

因Entamoeba spp感染。已知会引起灵长类动物（组织溶组织阿米巴）和蛇（入侵阿米巴）的严重疾病，但没有详细描述与Entamoeba spp相关的病理。两栖动物感染。2014年，与一种新的Entamoeba物种有关的大肠杆菌病暴发，在澳大利亚北部地区的自由放养的蟾蜍蟾蜍中引发临床疾病和身体状况不佳。在这里，我们描述与爆发有关的大体病理学，组织学和临床病理学。这项研究比较了25只蟾蜍，它们具有浸润性肠病，被定义为组织内组织学上可见的变形虫；还有12只蟾蜍没有浸润性肠病。总体而言，受影响的蟾蜍的结肠浆膜脉管系统充血程度较轻，肠壁增厚不一，对出血性结肠内容物呈血清型。组织学上，浸润性肠病主要表现为中度至重度，增生性至溃疡性结肠炎。小肠在25个蟾蜍中有10个受到感染，而25个蟾蜍中有5个也有胃部病变。变形虫与Entamoeba sp。的形态一致。蟾蜍通常与粘膜上皮混合在一起，经常沿着基底膜，只有5只蟾蜍更深地侵入浅表固有层。具有侵袭性肠病的蟾蜍具有嗜中性粒细胞增多，单核细胞增多和淋巴细胞减少症，因此嗜中性粒细胞与淋巴细胞的比率升高，提示炎症性和/或应激性白细胞检查。溃疡性结肠炎均有不同程度的增生。小肠在25个蟾蜍中有10个受到感染，而25个蟾蜍中有5个也有胃部病变。变形虫与Entamoeba sp。的形态一致。蟾蜍通常与粘膜上皮混合在一起，通常沿着基底膜，只有5只蟾蜍更深地侵入浅表固有层。具有侵袭性肠病的蟾蜍具有嗜中性粒细胞增多，单核细胞增多和淋巴细胞减少症，因此嗜中性粒细胞与淋巴细胞的比率升高，提示炎症性和/或应激性白细胞检查。溃疡性结肠炎的增生程度不同。小肠在25个蟾蜍中有10个受到感染，而25个蟾蜍中有5个也有胃部病变。变形虫与Entamoeba sp。的形态一致。蟾蜍通常与粘膜上皮混合在一起，通常沿着基底膜，只有5只蟾蜍更深地侵入浅表固有层。具有侵袭性肠病的蟾蜍具有嗜中性粒细胞增多，单核细胞增多和淋巴细胞减少症，因此嗜中性粒细胞与淋巴细胞的比率升高，提示炎症性和/或应激性白细胞检查。仅5只蟾蜍就更深地侵入浅表固有层。具有侵袭性肠病的蟾蜍具有嗜中性粒细胞增多，单核细胞增多和淋巴细胞减少症，因此嗜中性粒细胞与淋巴细胞的比率升高，提示炎症性和/或应激性白细胞检查。仅5只蟾蜍就更深地侵入浅表固有层。具有侵袭性肠病的蟾蜍具有嗜中性粒细胞增多，单核细胞增多和淋巴细胞减少症，因此嗜中性粒细胞与淋巴细胞的比率升高，提示炎症性和/或应激性白细胞检查。