Point-of-care (POC) detection and diagnostic platforms provide critical information about health and safety conditions in austere and resource-limited settings in which medical, military, and disaster relief operations are conducted. In this work, low-cost paper materials commonly used in POC devices are coated with liquid-infused polymer surfaces and folded to produce geometries that precisely localize complex liquid samples undergoing concentration by evaporation. Liquid-infused polymer surfaces were fabricated by infusing silicone-coated paper with a chemically compatible polydimethylsiloxane oil to create a liquid overlayer. Tests on these surfaces showed no remaining bacterial cells after exposure to a sliding droplet containing a concentrated solution of Escherichia coli or Staphylococcus aureus, while samples without a liquid layer showed adhesion of both microdroplets and individual bacterial cells. Folding of the paper substrates with liquid-infused polymer surfaces into several functional 3D geometries enabled a clean separation and simultaneous concentration of a liquid containing rhodamine dye into discrete, predefined locations. When used with bacteria, which are known for their ability to adhere to nearly any surface type, functional geometries with liquid-infused polymer surfaces concentrated the cells at levels significantly higher than geometries with dry control surfaces. These results show the potential of synergistically combining paper-based materials with liquid-infused polymer surfaces for the manipulation and handling of complex samples, which may help the future engineering of POC devices.

中文翻译：

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将折叠纸的几何形状与注入液体的聚合物表面相结合，以浓缩和定位细菌溶液。

即时医疗点（POC）检测和诊断平台提供有关在执行医疗，军事和救灾行动的严峻和资源有限的环境中有关健康和安全状况的重要信息。在这项工作中，通常在POC设备中使用的低成本纸材料上涂有注入液体的聚合物表面，然后折叠以产生可精确定位经过蒸发浓缩的复杂液体样品的几何形状。液体注入的聚合物表面是通过将有机硅涂布的纸与化学相容性的聚二甲基硅氧烷油注入以形成液体覆盖层而制成的。在这些表面上进行的测试表明，暴露于含有大肠杆菌或金黄色葡萄球菌浓缩液的滑动液滴后，没有残留细菌细胞，而没有液层的样品则表现出微滴和单个细菌细胞的粘附。将具有液体注入的聚合物表面的纸质基材折叠成几种功能性3D几何形状，可以将含若丹明染料的液体进行干净分离，并将其同时浓缩到离散的预定位置。当与细菌结合使用时，细菌以其几乎能粘附于任何表面类型的能力而闻名，具有液体注入聚合物表面的功能性几何结构将细胞浓缩的水平明显高于具有干燥对照表面的几何结构。这些结果表明，将纸质材料与注入液体的聚合物表面协同组合用于处理和处理复杂样品的潜力，这可能有助于POC设备的未来工程设计。