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Felis catus Papillomavirus Types 1, 2, 3, 4, and 5 in Feline Bowenoid in Situ Carcinoma: An In Situ Hybridization Study.
Veterinary Pathology ( IF 2.4 ) Pub Date : 2019-07-04 , DOI: 10.1177/0300985819859874
Marta Vascellari 1 , Maurizio Mazzei 2 , Claudia Zanardello 1 , Erica Melchiotti 1 , Francesco Albanese 3 , Mario Forzan 2 , Marta Francesca Croce 2 , Alberto Alberti 4 , Francesca Abramo 2
Affiliation  

Several studies based on histopathology or molecular investigations suggest a causal relation between Felis catus papillomavirus (FcaPV-2) infection and bowenoid in situ carcinoma (BISC) in cats. Nevertheless, data on distribution of viral DNA for different F. catus papillomavirus types (FcaPV-1, 2, 3, 4, 5) in precancerous skin lesions are lacking. In this study, incisional and excisional skin biopsies from 18 cats with BISC were investigated for the presence of FcaPV DNA by quantitative polymerase chain reaction (qPCR) and chromogenic in situ hybridization (CISH) using specific probes to detect each of the FcaPVs that have been identified so far. By qPCR analysis, 15 of 18 samples were positive for FcaPV-2, 2 were positive for FcaPV-4, and 1 sample was negative for all FcaPVs studied. Two cases were positive for FcaPV-5 by qPCR only. FcaPV-1 and FcaPV-3 were not detected by either method. CISH positivity for FcaPV-2 and FcaPV-4 was 100% concordant with qPCR. FcaPV-2 CISH signal was observed as nuclear dots within grouped neoplastic keratinocytes in 12 BISCs and in the perilesional skin of 9 biopsies. In 3 of these 9 cases, the signal was not observed within the BISC. FcaPV-4 CISH positivity was detected only within BISCs in 2 cases. The overall rate of concordance for FcaPV detection between PCR and CISH was 97.8%. This study suggests that CISH is a reliable method to detect FcaPV-2 and FcaPV-4 infection in cats and provides useful information on the type, rate, and localization of infected cells.

中文翻译:

猫类原位癌中猫1、2、3、4和5型猫乳头瘤病毒:原位杂交研究。

基于组织病理学或分子研究的多项研究表明,猫的猫乳头状瘤病毒(FcaPV-2)感染与弓形原位癌(BISC)之间存在因果关系。然而,缺乏关于癌前皮肤损伤中不同类型的F. catus乳头瘤病毒(FcaPV-1、2、3、4、5)的病毒DNA分布的数据。在这项研究中,通过定量聚合酶链反应(qPCR)和显色原位杂交(CISH),使用特定探针对18只患有BISC的猫的切开和切除皮肤活检进行了调查,以了解FcaPV DNA的存在,以检测每种FcaPV。到目前为止确定。通过qPCR分析,在18个样本中,有15个样本的FcaPV-2阳性,2个样本的FcaPV-4阳性,1个样本的所有FcaPVs均为阴性。仅通过qPCR,有2例FcaPV-5阳性。两种方法均未检测到FcaPV-1和FcaPV-3。FcaPV-2和FcaPV-4的CISH阳性率与qPCR一致(100%)。FcaPV-2 CISH信号被观察为12个BISCs和9个活检组织的病灶周围皮肤中成组的肿瘤性角质形成细胞内的核点。在这9例病例中的3例中,BISC内未观察到信号。FcaPV-4 CISH阳性仅在BISC内部检测到2例。PCR和CISH之间FcaPV检测的总体一致性率为97.8%。这项研究表明,CISH是检测猫中FcaPV-2和FcaPV-4感染的可靠方法,并提供了有关感染细胞的类型,发生率和定位的有用信息。FcaPV-2 CISH信号被观察为12个BISCs和9个活检组织的病灶周围皮肤中成组的肿瘤性角质形成细胞内的核点。在这9例病例中的3例中,BISC内未观察到信号。FcaPV-4 CISH阳性仅在BISC内部检测到2例。PCR和CISH之间FcaPV检测的总体一致性率为97.8%。这项研究表明,CISH是检测猫中FcaPV-2和FcaPV-4感染的可靠方法,并提供了有关感染细胞的类型,发生率和定位的有用信息。FcaPV-2 CISH信号被观察为12个BISCs和9个活检组织的病灶周围皮肤中成组的肿瘤性角质形成细胞内的核点。在这9例病例中的3例中,BISC内未观察到信号。FcaPV-4 CISH阳性仅在BISC内部检测到2例。PCR和CISH之间FcaPV检测的总体一致性率为97.8%。这项研究表明,CISH是检测猫中FcaPV-2和FcaPV-4感染的可靠方法,并提供了有关感染细胞的类型,发生率和定位的有用信息。
更新日期:2019-11-01
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