Wogonin, a naturally occurring monoflavonoid extracted from the root of Scutellaria baicalensis Georgi, has been reported for its anti-oxidant activity. However, it is still unclear whether wogonin can inhibit oxidant-induced vascular permeability. In this study, we evaluated the effects of wogonin on H2O2-induced vascular permeability in human umbilical vein endothelial cells (HUVECs). We found that wogonin can suppress the H2O2-stimulated actin remodeling and albumin uptake of HUVECs, as well as transendothelial cell migration of the human breast carcinoma cell MDA-MB-231. The mechanism revealed that wogonin inhibited H2O2-induced phosphorylation of caveolin-1 (cav-1) associating with the suppression of stabilization of VE-cadherin and β-catenin. Moreover, wogonin repressed anisomycin-induced phosphorylation of p38, cav-1 and vascular permeability. These results suggested that wogonin could inhibit H2O2-induced vascular permeability by downregulating the phosphorylation of cav-1, and that it might have a therapeutic potential for the diseases associated with the development of both oxidant and vascular permeability.

中文翻译：

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Wogonin通过抑制小窝蛋白1的磷酸化来抑制H2O2诱导的血管通透性。

Wogonin是从黄cut根中提取的天然单黄酮，具有抗氧化作用。但是，尚不清楚沃戈宁是否可以抑制氧化剂引起的血管通透性。在这项研究中，我们评估了wogonin对H2O2诱导的人脐静脉内皮细胞（HUVECs）的血管通透性的影响。我们发现，wogonin可以抑制HVECs的H2O2刺激的肌动蛋白重塑和白蛋白摄取，以及人乳腺癌细胞MDA-MB-231的跨内皮细胞迁移。该机制揭示了沃戈宁抑制H2O2诱导的caveolin-1（cav-1）磷酸化，同时抑制VE-钙粘蛋白和β-连环蛋白的稳定。此外，wogonin抑制了茴香霉素诱导的p38磷酸化，cav-1和血管通透性。这些结果表明，wogonin可以通过下调cav-1的磷酸化来抑制H2O2诱导的血管通透性，并且对于与氧化剂和血管通透性发展相关的疾病可能具有治疗潜力。