Wogonin, a naturally occurring monoflavonoid extracted from Scutellariae radix, has been shown to possess tumor therapeutic potential in vitro and in vivo. However, the effects of wogonin on tumor cells invasion remains poorly understood. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activity of wogonin in MDA-MB-231 human breast carcinoma cells. Wogonin caused a concentration-dependent suppression of cell migration, adhesion and invasion. The mechanism revealed that wogonin significantly inhibited the expression and activity of both endogenous and phorbol-12-myristate-13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) potentially associating with the suppression of translocation of protein kinase C (PKC) δ and phosphorylation of extracellular signal-regulated kinase (ERK1/2). These results suggested that wogonin could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-9, the possible targets may be PKCδ and ERK1/2.

中文翻译：

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wogonin通过下调基质金属蛋白酶9的表达和活性来抑制人乳腺癌细胞的侵袭。

Wogonin是从黄cut中提取的天然单黄酮类化合物，已显示出在体内和体外具有肿瘤治疗潜力。然而，沃戈宁对肿瘤细胞侵袭的影响仍然知之甚少。在这项研究中，我们进行了体外实验，以研究wogonin对MDA-MB-231人乳腺癌细胞的抗侵袭和抗转移活性。Wogonin引起细胞迁移，粘附和侵袭的浓度依赖性抑制。该机制表明，wogonin显着抑制内源性和佛波12-肉豆蔻酸13-乙酸盐（PMA）诱导的基质金属蛋白酶9（MMP-9）的表达和活性，这可能与抑制蛋白激酶C（ PKC）δ和细胞外信号调节激酶（ERK1 / 2）的磷酸化。