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Persistent colonization of non-lymphoid tissue-resident macrophages by Stenotrophomonas maltophilia.
International Immunology ( IF 4.4 ) Pub Date : 2020-02-07 , DOI: 10.1093/intimm/dxz071
Ichiro Takahashi 1, 2 , Koji Hosomi 2 , Takahiro Nagatake 2 , Hirokazu Toubou 1 , Daiki Yamamoto 1 , Ikue Hayashi 1 , Yosuke Kurashima 3 , Shintaro Sato 3 , Naoko Shibata 3 , Yoshiyuki Goto 3 , Fumito Maruyama 4 , Ichiro Nakagawa 4 , Asaomi Kuwae 5 , Akio Abe 5 , Jun Kunisawa 2, 3, 6, 7 , Hiroshi Kiyono 3
Affiliation  

Accumulating evidence has revealed that lymphoid tissue-resident commensal bacteria (e.g. Alcaligenes spp.) survive within dendritic cells. We extended our previous study by investigating microbes that persistently colonize colonic macrophages. 16S rRNA-based metagenome analysis using DNA purified from murine colonic macrophages revealed the presence of Stenotrophomonas maltophilia. The in situ intracellular colonization by S. maltophilia was recapitulated in vitro by using bone marrow-derived macrophages (BMDMs). Co-culture of BMDMs with clinically isolated S. maltophilia led to increased mitochondrial respiration and robust IL-10 production. We further identified a 25-kDa protein encoded by the gene assigned as smlt2713 (recently renamed as SMLT_RS12935) and secreted by S. maltophilia as the factor responsible for enhanced IL-10 production by BMDMs. IL-10 production is critical for maintenance of the symbiotic condition, because intracellular colonization by S. maltophilia was impaired in IL-10-deficient BMDMs, and smlt2713-deficient S. maltophilia failed to persistently colonize IL-10-competent BMDMs. These findings indicate a novel commensal network between colonic macrophages and S. maltophilia that is mediated by IL-10 and smlt2713.

中文翻译:

嗜麦芽窄食单胞菌对非淋巴组织驻留巨噬细胞的持久定殖。

越来越多的证据表明,淋巴样组织驻留共生细菌(例如Alcaligenes spp。)在树突状细胞中存活。我们通过研究持续在结肠巨噬细胞中定殖的微生物来扩展我们以前的研究。使用从鼠科结肠巨噬细胞纯化的DNA进行的基于16S rRNA的基因组分析表明,嗜麦芽窄食单胞菌存在。通过使用骨髓衍生的巨噬细胞(BMDM)在体外概括了嗜麦芽孢杆菌的原位细胞内定植。BMDM与临床分离的嗜麦芽胞杆菌的共培养导致线粒体呼吸增加和IL-10产生旺盛。我们进一步鉴定了由分配为smlt2713(最近更名为SMLT_RS12935)的基因编码并由S分泌的25 kDa蛋白。嗜麦芽炎是导致BMDM产生IL-10增强的因素。IL-10的产生对于维持共生状况至关重要,因为在IL-10缺陷型BMDM中,嗜麦芽孢杆菌的细胞内定植受到损害,而smlt2713缺陷型嗜麦芽孢杆菌无法持久地定殖于IL-10的BMDM。这些发现表明结肠巨噬细胞和嗜麦芽肿链球菌之间的新型共鸣网络是由IL-10和smlt2713介导的。
更新日期:2019-11-01
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