当前位置: X-MOL 学术Biotechniques › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immunochromatographic detection of MPB64 secreted from active BCG by heating: toward same-day diagnosis of tuberculosis.
Biotechniques ( IF 2.7 ) Pub Date : 2019-04-08 , DOI: 10.2144/btn-2019-0020
Kazunari Nakaishi,Satoshi Watabe,Toshiyuki Kitagawa,Etsuro Ito

We propose a new detection method for a tuberculosis-specific protein, MPB64, obtained from active bacillus Calmette-Guérin (BCG) by heating. When BCG was included in solution at a concentration >2.75 × 104 CFU/ml, our method for collecting MPB64 through heating active BCG combined with an immunochromatographic assay detected active bacilli within 2.5 h. By contrast, a culture test, which is the gold standard for tuberculosis diagnosis, does not provide results for between 1 week and 2 months. The rapid tests based on PCR have some drawbacks, for example they detect DNA from both active and latent (or even dead) tubercle bacilli. Therefore, our method may pave the way toward detecting only active tubercle bacilli at a reasonable cost and providing same-day diagnosis.

中文翻译:

免疫色谱法检测加热时从活动性BCG分泌的MPB64:对当日结核病的诊断。

我们提出了一种新的检测结核病特异性蛋白MPB64的方法,该蛋白是通过加热从活性卡介苗(BCG)中获得的。当溶液中包含的BCG浓度> 2.75×10 4 CFU / ml时,我们通过加热活性BCG结合免疫色谱法收集MPB64的方法在2.5小时内检测到了活性细菌。相比之下,作为结核病诊断的金标准的培养试验在1周至2个月内未提供结果。基于PCR的快速测试存在一些缺陷,例如,它们可以检测活动和潜在(甚至死亡)结核菌的DNA。因此,我们的方法可能为以合理的成本仅检测活动性结核杆菌铺平道路,并提供当日诊断。
更新日期:2020-08-21
down
wechat
bug