Background: PDQeX is a novel, single-step DNA extraction method that purifies nucleic acid from sample in under 30 min. Materials & Methods: Six bacterial suspensions from species with different cell morphologies and growth optima were made. DNA from half the suspension was purified using PDQeX and the other half using a conventional column purification method. Sequencing and analyses using Ion PGM were performed, blinded to extraction method and species. Results: Genomes extracted with either method sequenced successfully. No significant sequence distribution biases were evident between PDQeX and column purification. Surveyed community preference suggested comparable performance between the two extraction methods. Conclusion: DNA prepared using the PDQeX performs as well for whole-genome sequencing as DNA purified using a conventional method, albeit much more rapidly.

中文翻译：

---

使用PDQeX从细菌培养物中快速提取适合NGS工作流程的DNA。

背景： PDQeX是一种新颖的单步DNA提取方法，可在30分钟内从样品中纯化核酸。材料与方法：制备了六种来自具有不同细胞形态和最佳生长条件的物种的细菌悬液。使用PDQeX纯化一半悬浮液中的DNA，使用常规柱纯化方法纯化另一半。使用离子PGM进行测序和分析，对提取方法和种类不了解。结果：用两种方法提取的基因组均成功测序。在PDQeX和柱纯化之间没有明显的序列分布偏差。社区偏好调查表明，两种提取方法的性能相当。结论： 使用PDQeX制备的DNA与使用常规方法纯化的DNA相比，在全基因组测序中的表现要好得多，尽管速度要快得多。