Background: Evidence of fungal contamination of cannabis plants during drying has raised concerns of potential mycotoxin contamination of leaves and flowers and subsequent contamination of derived edible cannabis products. Methods are, therefore, needed for routine monitoring of cannabis to ensure consumer safety consistent with long-standing controls for mycotoxins such as aflatoxins and ochratoxin A (OTA) in foodstuffs. Objective: To generate preliminary validation data to demonstrate fitness-for-purpose of methods for aflatoxins and OTA in cannabis and cannabis products. Methods: Extraction of solid matrices with acetonitrile–water (75 + 25) and direct analysis of energy drinks after dilution. Extracts were either passed manually though an immunoaffinity column (IAC) containing antibodies to both aflatoxins and OTA or were analyzed sequentially using an automated system with in-line reusable immunoaffinity cartridges for aflatoxins or OTA. In both cases, analysis was by LC with fluorescence detection. Results: Recoveries were in the range of 76–120% with relative SDs from 0.8 to 6.6% for aflatoxins and OTA spiked into cannabis dried leaves and flowers, hemp tea, oils, capsules, cookies, chocolate brownies, and an energy drink. Conclusions: The methods described in this paper are suitable for the cleanup of sample extracts of cannabis and cannabis products. Highlights: Manual and automated methods with IAC cleanup have been shown to be suitable for routine control of aflatoxins and OTA in cannabis and cannabis products.

中文翻译：

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使用多抗体免疫亲和柱或带有可重复使用的在线免疫亲和柱的自动化系统进行的LC荧光检测，通过LC荧光检测法分析大麻和大麻产品中的黄曲霉毒素和O曲毒素A。

背景：大麻在干燥过程中受到真菌污染的证据引起人们对叶子和花朵可能受到霉菌毒素污染以及衍生的可食用大麻产品随后污染的担忧。因此，需要对大麻进行常规监测的方法，以确保消费者的安全与对食品中霉菌毒素（如黄曲霉毒素和曲霉毒素A（OTA））的长期控制相一致。目的：产生初步验证数据，以证明大麻和大麻产品中黄曲霉毒素和OTA方法的适用性。方法：用乙腈-水（75 + 25）萃取固体基质，稀释后直接分析能量饮料。提取物通过含有抗黄曲霉毒素和OTA抗体的免疫亲和柱（IAC）手动通过，或使用具有在线可重复使用的黄曲霉毒素或OTA免疫亲和柱的自动化系统顺序分析。在这两种情况下，均通过具有荧光检测功能的LC进行分析。结果：黄曲霉毒素和OTA加标到大麻干叶和花朵，大麻茶，油，胶囊，饼干，巧克力布朗尼和能量饮料中，黄曲霉毒素和OTA的回收率在76–120％范围内，相对标准偏差从0.8至6.6％。结论：本文描述的方法适用于大麻和大麻产品样品提取物的净化。重点：具有IAC清除功能的手动和自动方法已被证明适合常规控制大麻和大麻产品中的黄曲霉毒素和OTA。