POGZ is a heterochromatin protein 1 α-binding protein and regulates gene expression. On the other hand, accumulating pieces of evidence indicate that the POGZ gene abnormalities are involved in various neurodevelopmental disorders. In this study, we prepared a specific antibody against POGZ, anti-POGZ, and carried out biochemical and morphological characterization with mouse brain tissues. Western blotting analyses revealed that POGZ is expressed strongly at embryonic day 13 and then gradually decreased throughout the brain development process. In immunohistochemical analyses, POGZ was found to be enriched in cerebrocortical and hippocampal neurons in the early developmental stage. The nuclear expression was also detected in Purkinje cells in cerebellum at postnatal day (P)7 and P15 but disappeared at P30. In primary cultured hippocampal neurons, while POGZ was distributed mainly in the nucleus, it was also visualized in axon and dendrites with partial localization at synapses in consistency with the results obtained in biochemical fractionation analyses. The obtained results suggest that POGZ takes part in the regulation of synaptic function as well as gene expression during brain development.

中文翻译：

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POGZ，一种负责神经发育障碍的基因，在小鼠大脑发育过程中的表达分析。

POGZ是异染色质蛋白1α-结合蛋白，并调节基因表达。另一方面，越来越多的证据表明POGZ基因异常与各种神经发育疾病有关。在这项研究中，我们制备了针对POGZ，抗POGZ的特异性抗体，并对小鼠脑组织进行了生化和形态学表征。蛋白质印迹分析表明，POGZ在胚胎第13天表达强烈，然后在整个大脑发育过程中逐渐降低。在免疫组织化学分析中，发现POGZ在发育早期富含脑皮质和海马神经元。在出生后第（P）7和P15天，小脑的Purkinje细胞中也检测到了核表达，但在P30天消失了。在原代培养的海马神经元中 虽然POGZ主要分布在细胞核中，但也可以在轴突和树突中观察到，突触部分定位，这与生化分级分析的结果一致。获得的结果表明，POGZ在大脑发育过程中参与了突触功能以及基因表达的调节。