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Cellular Dynamics during Spinal Cord Regeneration in Larval Zebrafish.
Developmental Neuroscience ( IF 2.9 ) Pub Date : 2019-08-08 , DOI: 10.1159/000500185
Consuelo Anguita-Salinas 1 , Mario Sánchez 1 , Rodrigo A Morales 1 , María Laura Ceci 1 , Diego Rojas-Benítez 1 , Miguel L Allende 2
Affiliation  

The study of spinal cord regeneration using diverse animal models, which range from null to robust regenerative capabilities, is imperative for understanding how regeneration evolved and, eventually, to treat spinal cord injury and paralysis in humans. In this study, we used electroablation to fully transect the spinal cord of zebrafish larvae (3 days postfertilization) and examined regeneration of the tissue over time. We used transgenic lines to follow immune cells, oligodendrocytes, and neurons in vivo during the entire regenerative process. We observed that immune cells are recruited to the injury site, oligodendrocytes progenitor cells (olig2-expressing cells) invade, and axons cross the gap generated upon damage from anterior to reinnervate caudal structures. Together with the recovery of cell types and structures, a complete reversal of paralysis was observed in the lesioned larvae indicating functional regeneration. Finally, using transplantation to obtain mosaic larvae with single-labeled neurons, we show that severed spinal axons exhibited varying regenerative capabilities and plasticity depending on their original dorsoventral position in the spinal cord.

中文翻译:

幼虫斑马鱼脊髓再生过程中的细胞动力学。

使用从无效到强大的再生能力的多种动物模型进行脊髓再生的研究,对于理解再生的发展方式以及最终治疗人类的脊髓损伤和麻痹至关重要。在这项研究中,我们使用电消融法完全横切了斑马鱼幼虫的脊髓(受精后3天),并检查了组织随时间的再生。在整个再生过程中,我们使用转基因品系追踪体内的免疫细胞,少突胶质细胞和神经元。我们观察到免疫细胞被募集到损伤部位,少突胶质细胞祖细胞(表达olig2的细胞)侵入,并且轴突穿过从前到后的尾神经结构损伤产生的间隙。连同细胞类型和结构的恢复,在病变幼虫中观察到麻痹完全逆转,表明功能恢复。最后,使用移植获得具有单标记神经元的镶嵌幼虫,我们显示,切断的脊髓轴突表现出不同的再生能力和可塑性,这取决于它们在脊髓中的原始背腹位置。
更新日期:2019-11-01
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