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Comparative Fingerprint Profiling of Unani Polyherbomineral (Safoof-e-Pathar Phori) Formulation by HPTLC, HPLC, and GC-MS.
Journal of AOAC INTERNATIONAL ( IF 1.6 ) Pub Date : 2020-06-19 , DOI: 10.5740/jaoacint.19-0286
Wasim Ahmad 1, 2 , Rabea Parveen 1, 3 , Mohd Mujeeb 3 , S M Arif Zaidi 4
Affiliation  

Background: Standardization of Unani formulations is essential to assess the quality and safety of drugs to establish their acceptance internationally as well as in nationally. Safoof-e-Pathar phori is a polyherbomineral formulation that contains six different plant and mineral constituents: Didymocarpus pedicellata, Dolichos biflorus, Rheum emodi, Raphanus sativus, potassium carbonate, and potassium nitrate. Objective: The objective of the study is to develop and establish the quality of Safoof-e-Pathar phori, used for antilithiatic activity using chromatographic analysis. Methods: This study involves comparative fingerprint profiling of an Unani polyherbomineral formulation with its plant constituents by high-performance TLC (HPTLC), HPLC, and GC-MS. The authenticity of the Unani polyherbomineral formulation using comparative HPLC and GC-MS fingerprint profiling was achieved by comparing the retention time of peaks matching with plant drugs. Results: In HPTLC, comparative fingerprinting was done on the basis of retardation factor values of compounds separated on HPTLC plates of the formulation and the single drugs. The relative retention times of the characteristic peaks in the HPLC fingerprinting were established for identification of the Unani poly-herbo-mineral formulation, whereas GC-MS was used for comparative fingerprint profiling of different compounds present in single drugs and in formulation through the National Institute of Standards and Technology and Wiley libraries using mass by charge ration (m/z) of detected compounds. Conclusions: The fingerprint analysis approach is the most potent tool for quality control of polyherbal medicines because of its accuracy and reliability, and it also helps in identification and authentication and prevents adulteration. Highlights: The developed chromatographic methods enable the quality and safety profile of the products.

中文翻译:

通过HPTLC,HPLC和GC-MS对Unani多矿物(Safoof-e-Pathar Phori)制剂进行比较指纹分析。

背景: Unani制剂的标准化对于评估药物的质量和安全性,以确立其在国际和国内的接受度至关重要。Safoof-e-Pathar phori是一种多植物矿物配方,包含六种不同的植物和矿物质成分:Didymocarpus pedicellata,Dolichos biflorus,Rheum emodi,Raphanus sativus,碳酸钾和硝酸钾。目的:本研究的目的是开发和建立Safoof-e-Pathar phori的质量,该色谱用于色谱分析的抗石斑活性。方法:这项研究涉及通过高性能TLC(HPTLC),HPLC和GC-MS对Unani多矿物组合及其植物成分进行指纹比较分析。通过比较色谱峰与植物药物的保留时间,可以得出使用比较HPLC和GC-MS指纹图谱分析的Unani多草药矿物制剂的真实性。结果:在HPTLC中,根据在制剂和单一药物的HPTLC板上分离的化合物的延迟因子值进行比较指纹分析。建立了HPLC指纹图谱中特征峰的相对保留时间以鉴定Unani多药矿物配方,而GC-MS用于比较单一药物中和通过国立研究所配制的不同化合物的指纹图谱标准和技术库以及Wiley库使用检测到的化合物的质荷比(m / z)。结论:指纹分析方法由于其准确性和可靠性而成为用于多草药的质量控制的最有效工具,它还有助于识别和验证并防止掺假。重点:先进的色谱方法可确保产品的质量和安全性。
更新日期:2020-06-19
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