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A Chrnb3-Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells.
genesis ( IF 1.5 ) Pub Date : 2019-05-14 , DOI: 10.1002/dvg.23305
Lucy E Drayson 1 , Jason W Triplett 1, 2
Affiliation  

The mechanisms by which retinal ganglion cells (RGCs) make specific connections during development is an intense area of research and have served as a model for understanding the general principles of circuit wiring. As such, genetic tools allowing for specific recombination in RGCs are critical to further our understanding of the cell‐specific roles of different genes during these processes. However, many RGC‐specific Cre lines have drawbacks, due to their broad expression in other cell types and/or retinorecipient regions or lack of expression in broad swaths of the retina. Here, we characterize a Cre BAC transgenic line driven by elements of the cholinergic receptor nicotinic beta 3 subunit (Chrnb3). We show that Cre expression is restricted to RGCs in the retina and sparsely expressed in the brain, importantly excluding retinorecipient regions. Furthermore, Chrnb3‐Cre mice label a wide variety of RGCs distributed throughout the retina and Cre activity is detected embryonically, shortly following RGC differentiation. Finally, we find that Chrnb3‐Cre‐labeled RGCs innervate multiple retinorecipient areas that serve both image‐forming and nonimage forming functions. Thus, this genetic tool will be of broad use to investigators studying the RGC‐specific contributions of genes to visual circuit development.

中文翻译:

Chrnb3-Cre BAC转基因小鼠品系,用于操纵视网膜神经节细胞中的基因表达。

视网膜神经节细胞(RGC)在发育过程中建立特定连接的机制是研究的重点领域,并已成为理解电路布线一般原理的模型。因此,允许在RGC中进行特定重组的遗传工具对于进一步了解这些过程中不同基因在细胞中的特定作用至关重要。但是,许多RGC特异的Cre系由于其在其他细胞类型和/或视网膜受体区域中的广泛表达或在视网膜广泛区域中的缺乏表达而具有缺陷。在这里,我们表征了胆碱能受体烟碱β3亚基(Chrnb3)驱动的Cre BAC转基因品系。我们表明,Cre表达仅限于视网膜中的RGC,在大脑中稀疏表达,重要的是不包括视网膜受体区域。此外,Chrnb3-Cre小鼠标记了分布在整个视网膜中的各种RGC,在RGC分化后不久就通过胚胎检测到Cre活性。最后,我们发现Chrnb3-Cre标记的RGC支配了多个视网膜受体区域,这些区域既具有成像功能又具有非成像功能。因此,该遗传工具将被广泛用于研究RGC特定基因对视觉电路发育的研究的研究人员。
更新日期:2019-05-14
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